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Abstract In this study, we investigate interstellar absorption lines along the line of sight toward the galactic low-mass X-ray binary Cygnus X-2. We combine absorption line data obtained from high-resolution X-ray spectra collected with the Chandra and XMM-Newton satellites, along with far-UV absorption lines observed by the Hubble Space Telescope’s (HST) Cosmic Origins Spectrograph (COS) instrument. Our primary objective is to understand the abundance and depletion of oxygen, iron, sulfur, and carbon. To achieve this, we have developed an analysis pipeline that simultaneously fits both the UV and X-ray data sets. This novel approach takes into account the line-spread function of HST/COS, enhancing the precision of our results. We examine the absorption lines of Feii, Sii, Cii, and Cipresent in the far-UV spectrum of Cygnus X-2, revealing the presence of at least two distinct absorbers characterized by different velocities. Additionally, we employCloudysimulations to compare our findings concerning the ionic ratios for the studied elements. We find that gaseous iron and sulfur exist in their singly ionized forms, Feiiand Sii, respectively, while the abundances of Ciiand Cido not agree with the Cloudy simulations of the neutral ISM. Finally, we explore discrepancies in the X-ray atomic data of iron and discuss their impact on the overall abundance and depletion of iron.