Search for: All records | NSF Public Access

Note: When clicking on a Digital Object Identifier (DOI) number, you will be taken to an external site maintained by the publisher. Some full text articles may not yet be available without a charge during the embargo (administrative interval).
What is a DOI Number?

Some links on this page may take you to non-federal websites. Their policies may differ from this site.

Supramolecular Assembly of His-Tagged Fluorescent Protein Guests within Coiled-Coil Peptide Crystal Hosts: Three-Dimensional Ordering and Protein Thermal Stability

https://doi.org/10.1021/acsbiomaterials.2c00155

Curtis, Ryan W. ; Scrudders, Kevin L. ; Ulcickas, James R. ; Simpson, Garth J. ; Low-Nam, Shalini T. ; Chmielewski, Jean ( May 2022 , ACS Biomaterials Science & Engineering)

Full Text Available
Formation of Microcages from a Collagen Mimetic Peptide via Metal-Ligand Interactions

https://doi.org/10.3390/molecules26164888

Gleaton, Jeremy ; Curtis, Ryan W. ; Chmielewski, Jean ( August 2021 , Molecules)

Here, the hierarchical assembly of a collagen mimetic peptide (CMP) displaying four bipyridine moieties is described. The CMP was capable of forming triple helices followed by self-assembly into disks and domes. Treatment of these disks and domes with metal ions such as Fe(II), Cu(II), Zn(II), Co(II), and Ru(III) triggered the formation of microcages, and micron-sized cup-like structures. Mechanistic studies suggest that the formation of the microcages proceeds from the disks and domes in a metal-dependent fashion. Fluorescently-labeled dextrans were encapsulated within the cages and displayed a time-dependent release using thermal conditions.
more » « less
Full Text Available
A comparison of the collagen triple helix and coiled‐coil peptide building blocks on metal ion‐mediated supramolecular assembly

https://doi.org/10.1002/pep2.24190

Curtis, Ryan W. ; Chmielewski, Jean ( September 2020 , Peptide Science)

Abstract
Metal ion‐mediated assembly of peptides is an intriguing method for developing novel biomaterials. In particular, peptide building blocks based on collagen triple helical and coiled‐coil peptides have been functionalized with metal‐binding ligands and assemble into hierarchical structures with diverse potential applications. This review outlines the use of metal‐mediated assembly with these supramolecular structures and highlights how changes in the building blocks lead to significant differences in structural morphologies, with the identity of the metal‐binding ligands and their placement on the building block as crucial aspects in the bottom‐up development of the assemblies.

more » « less