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Abstract Thermal properties of cryopreserved tissues are critically important to the biopreservation community, which continues to seek more effective ways to store biological samples for improved outcomes in organ transplants as well as to facilitate the preservation of a record of biodiversity. Here, we present a reusable thermal needle-type 3-omega method designed for in situ characterization of such tissues, as well as other soft materials. The 3-omega method is a classic thermal materials characterization technique, which has been integrated into a modified microfabricated neural probe. This enables the measurement to be robust to environmental and experimental factors in cryopreservation. We demonstrate the viability of such a sensor to measure thermal conductivity for amorphous and crystalline solid samples of biological tissues, as demonstrated on 3mm thick chicken liver. These measurements can also be used for differentiation of solid samples, which is of particular interest for studies involving the kinetic limits of amorphous solidification (vitrification). In this, we demonstrate the value of a packaged thermal sensor to advancing the thermal understanding of cryopreserved biological systems and other solid-liquid phase change systems.