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A host–guest based fluorescence sensor array can sense small differences in protein structure. The combination of three cavitand hosts and two fluorophores to form a 4-component array is sufficient to fully discriminate five structurally similar Ig protein isotypes. The array can be applied to recognize Ig deficiencies in serum, when combined with a Protein L-based extraction process, allowing analysis of immunodeficiency in a simpler, lower cost manner than tests that require multiple specific antibodies.more » « less
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Liu, Yang; Duan, Yaokai; Gill, Adam D.; Perez, Lizeth; Jiang, Qiaoshi; Hooley, Richard J.; Zhong, Wenwan (, Chemical Communications)A synergistic combination of a deep cavitand host, fluorophore guests and transition metal ions can be used to sense small molecule thiols of biological interest with good efficiency and selectivity in complex aqueous media.more » « less
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Liu, Yang; Wang, Yu-Min; Sedano, Sabrina; Jiang, Qiaoshi; Duan, Yaokai; Shen, Wen; Jiang, Jian-Hui; Zhong, Wenwan (, Chemical Communications)Encapsulation of ionic nanoparticles in a hydrogel microparticle, i.e. microgel, produces a target-stimulated probe for molecular detection. Selective reactive oxygen species (ROS) triggers the release of cations from the microgel which subsequently turn on the fluorogenic dyes to emit intense fluorescence, permiting rapid detection of ROS or ROS-producing molecules. The ROS-responsive microgel provides the advantages of simple fabrication, bright and stable signals, easy handling, and rapid response, carrying high promises in biomedical applications.more » « less
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