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Abstract In a Quark-Gluon Plasma (QGP), the fundamental building blocks of matter, quarks and gluons, are under extreme conditions of temperature and density. A QGP could exist in the early stages of the Universe, and in various objects and events in the cosmos. The thermodynamic and hydrodynamic properties of the QGP are described by Quantum Chromodynamics (QCD) and can be studied in heavy-ion collisions. Despite being a key thermodynamic parameter, the QGP temperature is still poorly known. Thermal lepton pairs (e⁺e⁻andμ⁺μ⁻) are ideal penetrating probes of the true temperature of the emitting source, since their invariant-mass spectra suffer neither from strong final-state interactions nor from blue-shift effects due to rapid expansion. Here we measure the QGP temperature using thermale⁺e⁻production at the Relativistic Heavy Ion Collider (RHIC). The average temperature from the low-mass region (in-mediumρ⁰vector-meson dominant) is (2.01 ± 0.23) × 10¹²K, consistent with the chemical freeze-out temperature from statistical models and the phase transition temperature from Lattice QCD. The average temperature from the intermediate mass region (above theρ⁰mass, QGP dominant) is significantly higher at (3.25 ± 0.60) × 10¹²K. This work provides essential experimental thermodynamic measurements to map out the QCD phase diagram and understand the properties of matter under extreme conditions.