Mosquito bites transmit a number of pathogens via salivary droplets deposited during blood-feeding, resulting in potentially fatal diseases. Little is known about the genomic content of these nanodroplets, including the transmission dynamics of live pathogens. Here we introduce Vectorchip, a low-cost, scalable microfluidic platform enabling high-throughput molecular interrogation of individual mosquito bites. We introduce an ultra-thin PDMS membrane which acts as a biting interface to arrays of micro-wells. Freely-behaving mosquitoes deposit saliva droplets by biting into these micro-wells. By modulating membrane thickness, we observe species-dependent differences in mosquito biting capacity, utilizable for selective sample collection. We demonstrate RT-PCR andmore »
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Free, publicly-accessible full text available August 1, 2023
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Free, publicly-accessible full text available July 1, 2023
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Bacterial hopanoid lipids are ubiquitous in the geologic record and serve as biomarkers for reconstructing Earth’s climatic and biogeochemical evolution. Specifically, the abundance of 2-methylhopanoids deposited during Mesozoic ocean anoxic events (OAEs) and other intervals has been interpreted to reflect proliferation of nitrogen-fixing marine cyanobacteria. However, there currently is no conclusive evidence for 2-methylhopanoid production by extant marine cyanobacteria. As an alternative explanation, here we report 2-methylhopanoid production by bacteria of the genus
Nitrobacter , cosmopolitan nitrite oxidizers that inhabit nutrient-rich freshwater, brackish, and marine environments. The model organismNitrobacter vulgaris produced only trace amounts of 2-methylhopanoids when grown in minimal medium or withmore » -
Abstract With the advance of particle accelerator and detector technologies, the neutrino physics landscape is rapidly expanding. As neutrino oscillation experiments enter the intensity and precision frontiers, neutrino–nucleus interaction measurements are providing crucial input. MINERvA is an experiment at Fermilab dedicated to the study of neutrino–nucleus interactions in the regime of incident neutrino energies from one to few GeV. The experiment recorded neutrino and antineutrino scattering data with the NuMI beamline from 2009 to 2019 using the Low-Energy and Medium-Energy beams that peak at 3GeV and 6GeV, respectively. This article reviews the broad spectrum of interesting nuclear and particle physicsmore »Free, publicly-accessible full text available December 1, 2022
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Free, publicly-accessible full text available November 1, 2022