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Abstract Seasonally flooded forests along tropical rivers cover extensive areas, yet the processes driving air‐water exchanges of radiatively active gases are uncertain. To quantify the controls on gas transfer velocities, we combined measurements of water‐column temperature, meteorology in the forest and adjacent open water, turbulence with an acoustic Doppler velocimeter, gas concentrations, and fluxes with floating chambers. Under cooling, measured turbulence, quantified as the rate of dissipation of turbulent kinetic energy (ε), was similar to buoyancy flux computed from the surface energy budget, indicating convection dominated turbulence production. Under heating, turbulence was suppressed unless winds in the adjacent open water exceeded 1 m/s. Gas transfer velocities obtained from chamber measurements ranged from 1 to 5 cm/hr and were similar to or slightly less than predicted using a turbulence‐based surface renewal model computed with measuredεandεpredicted from wind and cooling.