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Free, publicly-accessible full text available October 13, 2025
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We recently reported that p28, one of the two turnip crinkle virus (TCV) replication proteins, trans-complemented a defective TCV lacking p28, yet repressed the replication of another TCV replicon encoding wildtype p28 (Zhang et al., 2017). Here we show that p88, the TCV-encoded RNA-dependent RNA polymerase, likewise trans-complemented a p88-defective TCV replicon, but repressed one encoding wild-type p88. Surprisingly, lowering p88 protein levels enhanced trans-complementation, but weakened repression. Repression by p88 was not simply due to protein over-expression, as deletion mutants missing 127 or 224 N-terminal amino acids accumulated to higher levels but were poor repressors. Finally, both trans-complementation and repression by p88 were accompanied by preferential accumulation of subgenomic RNA2, and a novel class of small TCV RNAs. Our results suggest that repression of TCV replication by p88 may manifest a viral mechanism that regulates the ratio of genomic and subgenomic RNAs based on p88 abundance.more » « less
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We recently reported that p28, one of the two turnip crinkle virus (TCV) replication proteins, trans-complemented a defective TCV lacking p28, yet repressed the replication of another TCV replicon encoding wildtype p28 (Zhang et al., 2017). Here we show that p88, the TCV-encoded RNA-dependent RNA polymerase, likewise trans-complemented a p88-defective TCV replicon, but repressed one encoding wild-type p88. Surprisingly, lowering p88 protein levels enhanced trans-complementation, but weakened repression. Repression by p88 was not simply due to protein over-expression, as deletion mutants missing 127 or 224 N-terminal amino acids accumulated to higher levels but were poor repressors. Finally, both trans-complementation and repression by p88 were accompanied by preferential accumulation of subgenomic RNA2, and a novel class of small TCV RNAs. Our results suggest that repression of TCV replication by p88 may manifest a viral mechanism that regulates the ratio of genomic and subgenomic RNAs based on p88 abundance.more » « less
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How bio-membranes are self-organized to perform their functions remains a pivotal issue in biological and chemical science. Understanding the self-assembly principles of lipid-like molecules hence becomes crucial. Here we report the meso-structural evolution of amphiphilic sphere-rod conjugates (giant lipids), and study the roles of geometric parameters (head-tail ratio and cross-section area) during this course. As a prototype system, giant lipids resemble natural lipidic molecules by capturing their essential features including head-tail configuration, monodispersed molecular weight distribution and minor interpenetration of hydrophobic tails. We demonstrate the self-assembly behavior of two categories of giant lipids (I-shape and T-shape, a total of 8 molecules). A rich variety of meso-structures are constructed in solution state and their molecular packing models are rationally understood. We streamline the driving forces of morphological evolution from both geometric and thermodynamic perspective. Giant lipids recast the phase behavior of both linear and branched lipidic molecules to certain degree, while the abundant self-assembled morphologies reveal distinct physiochemical behaviors when geometric parameters deviate from natural analogues.more » « less
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A bstract Diboson production in association with jets is studied in the fully leptonic final states, pp → (Z/
γ *)(Z/γ *) + jets → 2ℓ 2ℓ ′ + jets, (ℓ ,ℓ ′ = e orμ ) in proton-proton collisions at a center-of-mass energy of 13 TeV. The data sample corresponds to an integrated luminosity of 138 fb− 1collected with the CMS detector at the LHC. Differential distributions and normalized differential cross sections are measured as a function of jet multiplicity, transverse momentump T, pseudorapidityη , invariant mass and ∆η of the highest-p Tand second-highest-p Tjets, and as a function of invariant mass of the four-lepton system for events with various jet multiplicities. These differential cross sections are compared with theoretical predictions that mostly agree with the experimental data. However, in a few regions we observe discrepancies between the predicted and measured values. Further improvement of the predictions is required to describe the ZZ+jets production in the whole phase space.Free, publicly-accessible full text available October 1, 2025 -
A bstract A search for Higgs boson pair (HH) production in association with a vector boson V (W or Z boson) is presented. The search is based on proton-proton collision data at a center-of-mass energy of 13 TeV, collected with the CMS detector at the LHC, corresponding to an integrated luminosity of 138 fb
− 1. Both hadronic and leptonic decays of V bosons are used. The leptons considered are electrons, muons, and neutrinos. The HH production is searched for in the decay channel. An observed (expected) upper limit at 95% confidence level of VHH production cross section is set at 294 (124) times the standard model prediction. Constraints are also set on the modifiers of the Higgs boson trilinear self-coupling,$$ \textrm{b}\overline{\textrm{b}}\textrm{b}\overline{\textrm{b}} $$ k λ , assumingk 2V= 1, and vice versa on the coupling of two Higgs bosons with two vector bosons,k 2V. The observed (expected) 95% confidence intervals of these coupling modifiers are− 37.7 <k λ < 37.2 (− 30.1 <k λ < 28.9) and− 12.2 <k 2V< 13.5 (− 7.2 <k 2V< 8.9), respectively.Free, publicly-accessible full text available October 1, 2025 -
Free, publicly-accessible full text available October 1, 2025