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Creators/Authors contains: "Gustus, Kymberly C."

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  1. Abstract

    Adult neurogenesis is necessary for proper cognition and behavior, however, the mechanisms that underlie the integration and maturation of newborn neurons into the pre‐existing hippocampal circuit are not entirely known. In this study, we sought to determine the role of action potential (AP)‐dependent synaptic transmission by adult‐generated dentate granule cells (DGCs) in their survival and function within the existing circuitry. We used a triple transgenic mouse (NestinCreERT2:Snap25fl/fl: tdTomato) to inducibly inactivate AP‐dependent synaptic transmission within adult hippocampal progenitors and their progeny. Behavioral testing in a hippocampal‐dependent A/B contextual fear‐discrimination task revealed impaired discrimination learning in mice harboring SNAP‐25‐deficient adult‐generated dentate granule cells (DGCs). Despite poor performance on this neurogenesis‐dependent task, the production and survival of newborn DGCs was quantitatively unaltered in tamoxifen‐treated NestinCreERT2:Snap25fl/fl: tdTomato SNAP compared to tamoxifen‐treated NestinCreERT2:Snap25wt/wt: tdTomato control mice. Although SNAP‐25‐deficient adult DGCs displayed a small but statistically significant enhancement in proximal dendritic branching, their overall dendritic length and distal branching complexity was unchanged. SNAP‐25‐deficient newborn DGCs also displayed robust efferent mossy fiber output to CA3, with normal linear density of large mossy fiber terminals (LMTs). These studies suggest that AP‐dependent neurotransmitter release by newborn DGCs is not essential for their survival or rudimentary structural maturation within the adult hippocampus.

     
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