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Constraints on the state of stress in the lithosphere are fundamental to understanding a breadth of geological phenomena. Paleo-stresses are generally estimated using microstructural elements for which there are experimentally calibrated relationships with applied stress, with an emphasis on recrystallised grain-size piezometers. However, it is often difficult to clearly distinguish newly recrystallised grains from the relict matrix. Furthermore, these grain-size piezometers are only applicable to rocks consisting of a single mineral. An alternative proxy for paleo-stress in polymineralic rocks is the average subgrain size. Unfortunately, estimates of subgrain size differ significantly among different measurement methods, and therefore, piezometers must be individually calibrated for the method used. Existing subgrain-size piezometers are based on calibrations using optical or transmission electron microscopy. We use electron backscatter diffraction (EBSD), a common method of subgrain-boundary characterisation, to calibrate subgrain-size piezometers for both olivine and quartz. To test the application of our olivine subgrain-size piezometer to polymineralic rocks, we deformed synthetic mixtures of olivine and orthopyroxene. Experiments were conducted using a Deformation-DIA apparatus at beamline 6BM-B Advanced Photon Source, Argonne National Laboratory. These experiments offer the unique possibility of simultaneously deforming the sample and measuring the average stresses within each phase using X-ray diffraction, before applyingmore »