Search for: All records

Abstract Although DNA methylation primarily represses TEs, it also represses select genes that are methylated in plant body tissues but demethylated by DNA glycosylases (DNGs) in endosperm or pollen. Either one of two DNGs, MATERNAL DEREPRESSION OF R1 (MDR1) or DNG102, is essential for pollen viability in maize. Using single-pollen mRNA sequencing on pollen-segregating mutations in both genes, we identify 58 candidate DNG target genes that account for 11.1% of the wild-type transcriptome but are silent or barely detectable in other tissues. They are unusual in their tendency to lack introns but even more so in their TE-like methylation (teM) in coding DNA. The majority have predicted functions in cell wall modification, and they likely support the rapid tip growth characteristic of pollen tubes. These results suggest a critical role for DNA methylation and demethylation in regulating maize genes with the potential for extremely high expression in pollen but constitutive silencing elsewhere. 

Abstract Millimeter-wave refracting telescopes targeting the degree-scale structure of the cosmic microwave background (CMB) have recently grown to diffraction-limited apertures of over 0.5 m. These instruments are entirely housed in vacuum cryostats to support their subkelvin bolometric detectors and to minimize radiative loading from thermal emission due to absorption loss in their transmissive optical elements. The large vacuum window is the only optical element in the system at ambient temperature, and therefore minimizing loss in the window is crucial for maximizing detector sensitivity. This motivates the use of low-loss polymer materials and a window as thin as practicable. However, the window must simultaneously meet the requirement to keep a sufficient vacuum, and therefore must limit gas permeation and remain mechanically robust against catastrophic failure under pressure. We report on the development of extremely thin composite polyethylene window technology that meets these goals. Two windows have been deployed for two full observing seasons on the BICEP3 and the 150 GHz BICEP Array receiver CMB telescopes at the South Pole. On BICEP3, the window has demonstrated a 6% improvement in detector sensitivity. 

We present component-separated polarization maps of the cosmic microwave background (CMB) and Galactic thermal dust emission, derived using data from the BICEP/Keck experiments through the 2018 observing season and Planck. By employing a maximum-likelihood method that utilizes observing matrices, we produce unbiased maps of the CMB and dust signals. We outline the computational challenges and demonstrate an efficient implementation of the component map estimator. We show methods to compute and characterize power spectra of these maps, opening up an alternative way to infer the tensor-to-scalar ratio from our data. We compare the results of this map-based separation method with the baseline BICEP/Keck analysis. Our analysis demonstrates consistency between the two methods, finding an 84% correlation between the pipelines.