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Holguin, Jennifer ; Collins, Scott L ; McLaren, Jennie R ( , Environmental Data Initiative)Predicted climate change extremes, such as severe and prolonged drought, may profoundly impact biogeochemical processes like carbon and nitrogen cycling in water-limited ecosystems. To increase our understanding of how extreme climate events impact belowground ecosystem processes, we investigated the effects of five years of severe growing season drought and two-month delay in monsoon precipitation on belowground productivity and biogeochemical processes in two semi-arid grasslands. This experiment takes place during the fifth year of the Extreme Drought in Grassland Experiment (EDGE) at the Sevilleta National Wildlife Refuge (SNWR), a Long-Term Ecological Research in central New Mexico, USA. The two grassland sites a Chihuahuan Desert grassland dominated by Bouteloua eriopoda and Great Plains grassland dominated by B. gracilis are ~5km apart in the SWNR. The EDGE platform was established in the spring of 2012 (pre-treatment). Each site contains three treatments (ten replicates): ambient rainfall, extreme growing season drought, and delayed monsoon. The extreme drought treatment reduces growing season rainfall (April through September) each year by 66%, which equates to a 50% reduction of annual precipitation while maintaining natural precipitation patterns. There are 10 replicates per treatment within each site. All plots are 3 x 4 m in size and are paired spatially into blocks with treatments assigned randomly within a block. We measured an array of belowground and biogeochemical variables. Each variable was measured either once, twice, or three times (specific information on sampling scheme for each measured variable in methods section). Belowground net primary productivity, standing crop root biomass, total organic carbon, and total nitrogen were measured once. Extractable organic carbon, extractable total nitrogen, microbial biomass carbon, microbial biomass nitrogen and extracellular enzymes were measured twice. Available soil nitrate, available soil ammonium, and available soil phosphate were measured three times.more » « less