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ABSTRACT Organisms may simultaneously face thermal, desiccation and nutritional stress under climate change. Understanding the effects arising from the interactions among these stressors is relevant for predicting organisms' responses to climate change and for developing effective conservation strategies. Using both dynamic and static protocols, we assessed for the first time how sublethal desiccation exposure (at 16.7%, 50.0% and 83.3% of LD50) impacts the heat tolerance of foragers from two social bee species found on the Greek island of Lesbos: the managed European honey bee, Apis mellifera, and the wild, ground-nesting sweat bee Lasioglossum malachurum. In addition, we explored how a short-term starvation period (24 h), followed by a moderate sublethal desiccation exposure (50% of LD50), influences honey bee heat tolerance. We found that neither the critical thermal maximum (CTmax) nor the time to heat stupor was significantly impacted by sublethal desiccation exposure in either species. Similarly, starvation followed by moderate sublethal desiccation did not affect the average CTmax estimate, but it did increase its variance. Our results suggest that sublethal exposure to these environmental stressors may not always lead to significant changes in bees' heat tolerance or increase vulnerability to rapid temperature changes during extreme weather events, such as heat waves. However, the increase in CTmax variance suggests greater variability in individual responses to temperature stress under climate change, which may impact colony-level performance. The ability to withstand desiccation may be impacted by unmeasured hypoxic conditions and the overall effect of these stressors on solitary species remains to be assessed. 

Synopsis Animal-mediated pollination is one of the most ecologically and economically important mutualisms and serves as a remarkable example of cross-kingdom communication and coevolution. Unfortunately, pollinators, plants, and the interactions between them are threatened in the Anthropocene. While pollination emerges from interactions across biological scales, existing research and expertise have developed in distinct silos reflecting traditional fields of study such as ecology, plant physiology, neuroethology, etc. This forward-looking review and perspective is a culmination of the “Plant-pollinator interactions in the Anthropocene” symposium at the 2025 Society for Integrative and Comparative Biology meeting, which collected expertise across these disciplinary silos to identify pressing questions our community needs to tackle in the next decade. In this perspective piece, we argue that an integrative, organismally informed systems approach is critical to unraveling the complexity of how plant-pollinator relationships are impacted by dynamic anthropogenic stressors. Specifically, this calls for an intentional and iterative integration of holistic modeling studies with empirical studies. Modeling the emergent properties driven by organismal interactions in pollination systems can identify impactful variables; this in turn should drive design of empirical studies that elucidate how organisms respond to changing environments in the context of those impactful variables, feeding back into improved models. Repetition of this process will allow better predictive power over pollination stability in changing landscapes. Finally, we consider both existing barriers to this integration, as well as emerging opportunities (such as new technologies) that can help bridge across traditional fields. 

Bees are essential pollinators and understanding their ability to cope with extreme temperature changes is crucial for predicting their resilience to climate change, but studies are limited. We measured the response of the critical thermal maximum (CTMax) to short-term acclimation in foragers of six bee species from the Greek island of Lesvos, which differ in body size, nesting habit, and level of sociality. We calculated the acclimation response ratio as a metric to assess acclimation capacity and tested whether bees’ acclimation capacity was influenced by body size and/or CTMax. We also assessed whether CTMax increases following acute heat exposure simulating a heat wave. Average estimate of CTMax varied among species and increased with body size but did not significantly shift in response to acclimation treatment except in the sweat bee Lasioglossum malachurum. Acclimation capacity averaged 9% among species and it was not significantly associated with body size or CTMax. Similarly, the average CTMax did not increase following acute heat exposure. These results indicate that bees might have limited capacity to enhance heat tolerance via acclimation or in response to prior heat exposure, rendering them physiologically sensitive to rapid temperature changes during extreme weather events. These findings reinforce the idea that insects, like other ectotherms, generally express weak plasticity in CTMax, underscoring the critical role of behavioral thermoregulation for avoidance of extreme temperatures. Conserving and restoring native vegetation can provide bees temporary thermal refuges during extreme weather events.