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The correct interpretation of the result from a point-of-care device is crucial for an accurate and rapid diagnosis to guide subsequent treatment. Lateral flow tests (LFTs) use a well-established format that was designed to simplify the user experience. However, the LFT device architecture is inherently limited to detecting analytes that can be captured by molecular recognition. Microfluidic paper-based analytical devices (µPADs), like LFTs, have the potential to be used in diagnostic applications at the point of care. However, µPADs have not gained significant traction outside of academic laboratories, in part, because they have often demonstrated a lack of homogeneous shape or color in signal outputs, which consequently can lead to inaccurate interpretation of results by users. Here, we demonstrate a new class of µPADs that form colorimetric signals at the interfaces of converging liquid fronts (i.e., lines) to control where colorimetric signals are formed without relying on capture techniques. We demonstrate our approach by developing assays for three classes of analytes—an ion, an enzyme, and a small molecule—to measure using iron (III), acetylcholinesterase, and lactate, respectively. Additionally, we show these devices have the potential to support multiplexed assays by generating multiple lines in a common readout zone. These results highlight the ability of this new paper-based device architecture to aid the interpretation of assays that create soluble products by using flow to constrain those colorimetric products in a familiar, line-format output. 

The intersection between the Mid-Atlantic Ridge and Iceland hotspot provides a natural laboratory where the composition and dynamics of Earth's upper mantle can be observed. Plume-ridge interaction drives variations in the melting regime, which result in a range of crustal types, including a series of V-shaped ridges (VSRs) and V-shaped troughs (VSTs) located south of Iceland. Mantle upwelling beneath Iceland dynamically supports regional bathymetry, and its variations may lead to changes in the height of oceanic gateways, which in turn control the flow of deep water on geologic timescales. Expeditions 384, 395C, and 395 recovered extensive successions of basaltic crust and thick (up to 1.3 km) overlying sediment cover, including successions through a number of contourite drifts of regional significance. Major, trace, and isotope geochemistry of basalts recovered during these expeditions will provide insight into spatial and temporal variations in mantle melting processes. Such analyses will provide data for testing the hypothesis that the Iceland plume thermally pulses on two timescales (5–10 and ~30 Ma), leading to fundamental changes in crustal architecture. This idea will be tested against alternative hypotheses involving propagating rifts and buoyant mantle upwelling. The rapidly accumulated sediments of contourite drifts have the potential to yield exceptional millennial-scale paleoceanographic records, including proxies for current strength, which is thought to be modulated by the dynamic support of the Greenland-Scotland Ridge, an oceanic gateway of global import. The recovered sediments also provide a record of subarctic climate change stretching back to the latest Eocene, including the long-term evolution of the Greenland ice sheet, critical intervals of Miocene and Pliocene warmth, the intensification of Northern Hemisphere glaciation, and Pleistocene millennial-scale variability. The objectives of Expeditions 395, 395C, and 384 are to explore the relationships between deep Earth processes, ocean circulation, and climate. These objectives were addressed by recovering sediment and basement cores from six sites, completed across three expeditions. Sites U1555 and U1563 are located at a VST/VSR pair nearest to the Reykjanes Ridge, on ~2.8 and 5.2 My old crust, respectively. Sites U1554 and U1562 are located in Björn drift above a VST/VSR pair, on ~12.4 and 14.2 My old crust, respectively. Site U1564 is located in Gardar drift above 32.4 My old oceanic crust that is devoid of V-shaped features. Finally, Site U1602 is located on the eastern Greenland margin above crust that is estimated to be Eocene in age and thus formed during the initial separation of Greenland from Scandinavia. Considered together, the sediments, basalts, and vast array of measurements collected during Expeditions 395, 395C, and 384 will provide a major advance in our understanding of mantle dynamics and the linked nature of Earth's interior, oceans, and climate.