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The Lamprey River watershed, located in southeastern New Hampshire (USA), drains 554 km² of low elevation land before discharging into the Great Bay Estuary (Wymore and others 2021). The watershed is classified as suburban with mixed land-use that includes forests (73%), wetlands (10%), development (7%), and agriculture (NOAA Coastal Change Analysis Program 2016). We selected four distinct sites within the watershed to capture the dynamics of both tributaries and the mainstem, while accounting for variations in land use, land cover, and nutrient availability. Wednesday Hill Brook (WHB) is a 1st-order stream that drains a residential landscape and has the highest NO3 concentrations among the sites due to a high density of septic systems (Flint and McDowell 2015). Dowst Cate Forest (DCF) is a 2nd-order stream draining a headwater wetland and forested landscape and is characterized by the highest concentrations of dissolved organic carbon (DOC). The two main stem Lamprey River sites, LMP72 and LMP73, exhibit moderate concentrations of both DOC and NO3 and are located approximately 1 km apart. LMP72 is located at a low-head run-of-river dam while LMP73 is free-flowing water downstream of the reservoir. Our dataset includes weekly water chemistry and dissolved gas data collected from April 2014 through May 2023, except for DCF where data collection ended in 2021, resulting in a total of 1,179 observations across the four different sites.