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In this report, the relative efficiency of cellulose nanocrystals (CNCs) and nanofibers (CNFs) to capture circulating tumor cells (CTCs) from the blood sample of head and neck cancer (HNC) patients was evaluated. Detection and enumeration of CTCs are critical for monitoring cancer progression. Both types of nanostructured cellulose were chemically modified with Epithelial Cell Adhesion Molecule (EpCAM) antibody and iron oxide nanoparticles. The EpCAM antibody facilitated the engagement of CTCs, promoting entrapment within the cellulose cage structure. Iron oxide nanoparticles, on the other hand, rendered the cages activatable via the use of a magnet for the capture and separation of entrapped CTCs. The efficiency of the network structures is shown in head and neck cancer (HNC) patients' blood samples. It was observed that the degree of chemical functionalization of hydroxyl groups located within the CNCs or CNFs with anti-EpCAM determined the efficiency of the system's interaction with CTCs. Further, our result indicated that inflexible scaffolds of nanocrystals interacted more efficiently with CTCs than that of the fibrous CNF scaffolds. Network structures derived from CNCs demonstrated comparable CTC capturing efficiency to commercial standard, OncoDiscover®. The output of the work will provide the chemical design principles of cellulosic materials intended for constructing affordable platforms for monitoring cancer progression in 'real time'. 

A novel additive manufacturing process utilizing the laminated object manufacturing (LOM) technology with woven natural fiber-reinforced biopolymer is investigated in this paper. Traditional synthetic composite materials are products from nonrenewable crude oil with limited end-of-life options, and therefore not environmentally friendly. The continuous woven natural fiber is used to significantly strengthen the mechanical properties of biocomposites and PLA biopolymer as the matrix made the material completely biodegradable. This is one of the promising replacements for synthetic composites in applications such as automotive panels, constructive materials, and sports and musical instruments. A LOM 3D printer prototype has been designed and built by the team using a laser beam in cutting the woven natural fiber reinforcement and molten PLA powder to bind layers together. Tensile and flexural properties of the LOM 3D printed biocomposites were measured using ASTM test standards and then compared with corresponding values measured from pure PLA specimens 3D printed through FDM. Improved mechanical properties from LOM 3D-printed biocomposites were identified by the team. SEM imaging was performed to identify the polymer infusing and fiber-matrix binding situations. This research took advantage of both the material and process’s benefits and combine them into one sustainable practice.