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Mitochondria lack nucleotide excision repair; however, mitochondrial DNA (mtDNA) is resistant to mutation accumulation following DNA damage. These observations suggest additional damage sensing or protection mechanisms. Transcription Factor A, Mitochondrial (TFAM) compacts mtDNA into nucleoids and binds differentially to certain forms of DNA damage. As such, TFAM has emerged as a candidate for protecting mtDNA or sensing damage. To examine the possibilities that TFAM might protect DNA from damage or act as a damage sensing protein for irreparable forms of mtDNA damage, we used live-cell imaging and HeLa cell-based assays, atomic force microscopy (AFM), and high-throughput protein-DNA binding assays to characterize the binding properties of human TFAM to ultraviolet-C (UVC) irradiated DNA and the cellular consequences of UVC irradiation. Our cell data show increased TFAM mRNA after exposure and suggest an increase in mtDNA degradation without a loss in mitochondrial membrane potential that might trigger mitophagy. Our protein-DNA binding assays indicate a reduction in sequence specificity of TFAM following UVC irradiation and a redistribution of TFAM binding throughout the mitochondrial genome. Our AFM data show increased compaction of DNA by TFAM in the presence of damage. Despite the TFAM-mediated compaction of mtDNA in vitro, we do not observe any protective effect of increased TFAM protein on DNA damage formation in cells or in vitro. Increased TFAM protein did not alter levels of mtDNA damage over time after UVC exposure in vivo, but knockdown of TFAM did alter mtDNA damage levels in HeLa cells both at baseline and after UVC exposure. Taken together, these studies indicate that UVC-induced DNA damage alters TFAM binding and promotes compaction by TFAM in vitro. We hypothesize that TFAM may act as a damage sensing protein in vivo, sequestering damaged genomes to prevent mutagenesis by facilitating removal or suppression of replication. 

Abstract We explore the potential for repeat‐pass SAR Interferometry (InSAR) correlation to track volcanic activity on Venus' surface motivated by future SAR missions to Earth's sister planet. We use Hawai'i as a natural laboratory to test whether InSAR can detect lava flows assuming orbital and instrument parameters similar to that of a Venus mission. Hawai'i was chosen because lava flows are frequent, and well documented by the United States Geological Survey, and because Hawai'i is a SAR supersite, where space agencies have offered open radar data sets for analysis. These data sets have different wavelengths (L, C, and X bands), bandwidths, polarizations, look angles, and a variety of orbital baselines, giving opportunity to assess the suitability of parameters for detecting lava flows. We analyze data from ALOS‐2 (L‐band), Sentinel‐1 (C‐band), and COSMO‐SkyMed (X‐band) spanning 2018 and 2022. We perform SAR amplitude and InSAR correlation analysis over temporal baselines and perpendicular baselines similar to those of a Venus mission. Fresh lava flows create a sharp, noticeable decrease in InSAR correlation that persists indefinitely for images spanning the event. The same lava flows are not always visible in the corresponding amplitude images. Moreover, noticeable decorrelation persists in image pairs acquired months after the events due to post‐emplacement contraction of flows. Post‐emplacement effects are hypothesized to last longer on the Venusian surface, increasing the likelihood of detecting Venus lava flows using InSAR. We argue for further focus on repeat‐pass InSAR capabilities in upcoming Venus missions, to detect and quantify volcanic activity on Earth's hotter twin. 

Precipitation over Puerto Rico is expected to decline in coming decades, threatening an important source of surface water supply. However, earth system models neither resolve fine‐scale orographic impacts nor the radiative effects of Saharan dust aerosols on precipitation over the island's mountains. Here, we use the Weather Research and Forecasting model coupled with chemistry (WRF‐Chem) to downscale global climate model projections to a convection‐resolving 4‐km resolution for both dust and no‐dust scenarios. We compare the regional hydroclimate during three periods: historical (2014–2018), mid‐ (2045–2049) and end‐21st century (2085–2089), using output from an RCP8.5 simulation of the Community Earth System Model, version 1. For each period, the coarsest parent domain is simulated both with and without dust emission, transport and radiative effects, and the ensuing thermodynamic perturbations passed to the 4‐km domain via nesting. The downscaled output indicates that precipitation over eastern Puerto Rico is expected to decline through the end of the century, leading to an increase in consecutive dry days and a depletion of soil moisture that is evident across all seasons. The inclusion of dust exacerbates the drying signal during late summer when Saharan air outbreaks are active but ameliorates drying in other seasons relative to its historical simulation. Rainfall decreases rapidly by the mid‐21st century, and the declines plateau through 2100 when dust is included, whereas there is a modest rebound to wetter conditions when dust is ignored. Although the highest elevations exhibit resilience to future drying, there is a clearer drying signal at low elevations. 

Synopsis Salt marsh ecosystems are heavily reliant on ribbed mussel (Geukensia demissa) populations to aid in rapid recovery from droughts. The focus of this study was thus to document the effects of rising temperatures on ribbed mussel populations in a Georgia salt marsh. Seven lab and eight field experiments were used to assess the effects of current air temperatures on mussels at two high marsh (HM) sites with short and sparse cordgrass and one mid marsh (MM) site with tall and dense cordgrass. Field results in 2018 and 2019 indicate that ribbed mussels were experiencing extremely high temperatures for prolonged periods of time at the landlocked high marsh (LHM) site. In 2018, the highest temperature (54°C) and longest high temperature events, HTEs (58 days), that is, consecutive days with temperatures ≥40°C, were recorded at this site. When laboratory temperatures were increased from 20 to 36°C, mean heart rates increased by an average of 19 bpm for mussels from both high and MM sites respectively. When field temperatures rose from 20°C in April to 40°C in September 2019, mean heart rates increased by an average of 10 bpm for HM mussels and by 26.3 bpm for MM mussels. Under identical laboratory and field conditions, mean heart rates for mussels from the LHM site with the highest temperatures, increased by <1 bpm and 3.7 bpm respectively. Evidence of the potential role of shade on mussel aggregates was provided by examining whether mussels from the edge of mussel aggregates with little to no cordgrass for shade were more stressed than those living at the center of mussel aggregates. In the absence of shade, mean body temperatures for mussels at the edge of mussel aggregates were up to 8°C higher than for those living in the center underneath a dense tuft of cordgrass. Despite high body temperatures, mean heart rates and Hsp70 gene expression were lower for mussels living at the edges. This agrees with the strategy that during prolong exposure to high temperatures, mussels may reduce their heart rate to conserve energy and enhance survival. Alternatively, heat-stressed mussels at the edges of aggregates may not have the resources to express high levels of Hsp70. Increase in the frequency, intensity, and duration of HTEs may stress the physiological and biochemical function of mussel populations to the limit, dictate mussel aggregate size, and threaten the functionality of SE salt marshes. 

Paleomagnetic, rock magnetic, or geomagnetic data found in the MagIC data repository from a paper titled: PSV10: A Global Data Set for 0–10 Ma Time‐Averaged Field and Paleosecular Variation Studies 

Abstract The western North American Great Basin's Carlin-type deposits represent the largest accumulation of gold in the Northern Hemisphere. The controversy over their origins echoes the debate between Neptunists and Plutonists at the birth of modern geology: were the causative processes meteoric or magmatic? Sulfur isotopes have long been considered key to decoding metal cycling in the Earth's crust, but previous studies of Carlin-type pyrite lacked the spatial resolution to quantify differences among the numerous generations of sulfide mineralization. We developed a new dual-method, nanoscale approach to examine the fine-grained ore pyrite. The δ34S of the ore pyrite varies systematically with Au concentration at the nanoscale, indicating that both magmatic and meteoric fluids contributed during mineralization, but the magmas brought the gold. Repeated oscillations in fluid ratios upgraded the metal content, resulting in high gold endowment. Our results demonstrate that high-spatial-resolution studies are key to elucidate the spatiotemporal evolution of complex hydrothermal systems.