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  1. null (Ed.)
  2. null (Ed.)
    Plastics pollution represents a global environmental crisis. In response, microbes are evolving the capacity to utilize synthetic polymers as carbon and energy sources. Recently, Ideonella sakaiensis was reported to secrete a two-enzyme system to deconstruct polyethylene terephthalate (PET) to its constituent monomers. Specifically, the I. sakaiensis PETase depolymerizes PET, liberating soluble products, including mono(2-hydroxyethyl) terephthalate (MHET), which is cleaved to terephthalic acid and ethylene glycol by MHETase. Here, we report a 1.6 Å resolution MHETase structure, illustrating that the MHETase core domain is similar to PETase, capped by a lid domain. Simulations of the catalytic itinerary predict that MHETase follows the canonical two-step serine hydrolase mechanism. Bioinformatics analysis suggests that MHETase evolved from ferulic acid esterases, and two homologous enzymes are shown to exhibit MHET turnover. Analysis of the two homologous enzymes and the MHETase S131G mutant demonstrates the importance of this residue for accommodation of MHET in the active site. We also demonstrate that the MHETase lid is crucial for hydrolysis of MHET and, furthermore, that MHETase does not turnover mono(2-hydroxyethyl)-furanoate or mono(2-hydroxyethyl)-isophthalate. A highly synergistic relationship between PETase and MHETase was observed for the conversion of amorphous PET film to monomers across all nonzero MHETase concentrations tested. Finally, we compare the performance of MHETase:PETase chimeric proteins of varying linker lengths, which all exhibit improved PET and MHET turnover relative to the free enzymes. Together, these results offer insights into the two-enzyme PET depolymerization system and will inform future efforts in the biological deconstruction and upcycling of mixed plastics. 
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  3. Abstract

    Understanding and modeling variability of ground motion is essential for building accurate and precise ground motion prediction equations, which can net site‐specific characterization and reduced hazard levels. Here, we explore the spatial variability in peak ground velocity (PGV) at Sage Brush Flats along the San Jacinto Fault in Southern California. We use data from a dense array (0.6 × 0.6 km2, 1,108 geophones, station spacings 10–30 m) deployed in 2014 for ~1 month. These data offer an opportunity to study small‐scale variability in this region. We examine 38 earthquakes (2 ≤ ML ≤ 4.2) within 200 km of the array. Fault strands and a small basin impact the ground motions, producing PGV variations up to 22% of the mean and a 40% reduction inPandSwave near‐surface velocities. We find along‐fault rupture directivity, source, and path effects can increase PGVs by 167%. Surface PGV measurements exceed the colocated borehole station (depth at 148 m) PGV by factors of 3–10, confirming the impact on PGV from near‐surface fault structures, basins, topography, and amplifications from soft sediments. Consistently, we find high PGVs within the basin structure. A pair of colocated GaML2.6 events produce repeatable PGV values with similar spatial patterns. The average corner frequencies of these two events are 11–16 Hz, and viable measurements of stress drop can differ by 6.45 MPa. Within this small array, the PGV values are variable implying spatial extrapolation of PGV to regions of known faults and basins, even across a small area, should be done with caution.

     
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  4. Abstract

    Proper classification of nontectonic seismic signals is critical for detecting microearthquakes and developing an improved understanding of ongoing weak ground motions. We use unsupervised machine learning to label five classes of nonstationary seismic noise common in continuous waveforms. Temporal and spectral features describing the data are clustered to identify separable types of emergent and impulsive waveforms. The trained clustering model is used to classify every 1 s of continuous seismic records from a dense seismic array with 10–30 m station spacing. We show that dominate noise signals can be highly localized and vary on length scales of hundreds of meters. The methodology demonstrates the complexity of weak ground motions and improves the standard of analyzing seismic waveforms with a low signal‐to‐noise ratio. Application of this technique will improve the ability to detect genuine microseismic events in noisy environments where seismic sensors record earthquake‐like signals originating from nontectonic sources.

     
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