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  1. Free, publicly-accessible full text available September 1, 2022
  2. Students' academic learning, performance, and motivation are ongoing topics in engineering education. Those studies that have attempted to understand the mechanisms of motivation in authentic classroom settings and scenarios are few and limited to the methods used (e.g., self-reports, observations). This Work-in-Progress study explores the utility of electrodermal activity (EDA) and temperature sensors in accurately informing scholars about student performance during an exam in real-time. Correlations between each factor were analyzed. Initial results suggest that peripheral skin temperature has a weak, positive but significant correlation to exam question difficulty r=0.08; p<; 0.001). Also, electrodermal activity and temperature showed a weak,more »positive, but significant correlation (r=0.13; p<; 0.0010.05). The electrodermal activity showed a weak, positive, but significant correlation to exam question difficulty (r=0.16; p<; 0.0010.01). Also, skin temperature correlations with difficulty index (did not) changed across semesters (r=0.18; p<; 0.0010.001). We also developed a multiple regression model and found moderately significant relationships between EDA, difficulty index, and skin temperature (r=0.45; p<; 0.0010.05). The findings suggest that performance is tied to physiological responses among students during exam taking, indicating a possible connection between emotions and cognition via physiology.« less
  3. A bio-orthogonal chemistry-based approach for fluorescent labelling of ribosomal RNA is described. It involves an adenosine analogue modified with trans -cyclooctene and masked 5′-phosphate group using aryl phosphoramidate. The incorporation into rRNA has been confirmed using agarose gel electrophoresis, as well as a highly sensitive UHPLC-MS/MS method. Fluorescent labelling of rRNA has been achieved in live HeLa cells via an inverse electron demand Diels–Alder reaction with a tetrazine conjugated to an Oregon Green fluorophore. This communication describes the stepwise approach that led to the development and characterization of the probe. The results demonstrate a new strategy towards development of futuremore »fluorescent probes to investigate the biochemistry of nucleic acids.« less
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