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Delivery of therapeutic stem cells to treat bone tissue damage is a promising strategy that faces many hurdles to clinical translation. Among them is the design of a delivery vehicle which promotes desired cell behavior for new bone formation. In this work, we describe the use of an injectable microporous hydrogel, made of crosslinked gelatin microgels, for the encapsulation and delivery of human mesenchymal stem cells (MSCs) and compared it to a traditional nonporous injectable hydrogel. MSCs encapsulated in the microporous hydrogel showed rapid cell spreading with direct cell–cell connections whereas the MSCs in the nonporous hydrogel were entrapped by the surrounding polymer mesh and isolated from each other. On a per-cell basis, encapsulation in microporous hydrogel induced a 4 × increase in alkaline phosphatase (ALP) activity and calcium mineral deposition in comparison to nonporous hydrogel, as measured by ALP and calcium assays, which indicates more robust osteogenic differentiation. RNA-seq confirmed the upregulation of the genes and pathways that are associated with cell spreading and cell–cell connections, as well as the osteogenesis in the microporous hydrogel. These results demonstrate that microgel-based injectable hydrogels can be useful tools for therapeutic cell delivery for bone tissue repair.

 

Chemical anomalies in planet-hosting stars (PHSs) are studied in order to assess how the planetary nature and multiplicity affect the atmospheric chemical abundances of their host stars. We employ APOGEE DR17 to select thin-disk stars of the Milky Way, and crossmatch them with the Kepler Input Catalog to identify confirmed PHSs, which results in 227 PHSs with available chemical abundance ratios for six refractory elements. We also examine an ensemble of stars without planet signals, which are equivalent to the selected PHSs in terms of evolutionary stage and stellar parameters, to correct for Galactic chemical evolution effects, and derive the abundance gradient of refractory elements over the condensation temperature for the PHSs. Using the Galactic chemical evolution corrected abundances, we find that our PHSs do not show a significant difference in abundance slope from the stars without planets. However, when we examine the trends of the refractory elements of PHSs, based on the total number of their planets and their planet types, we find that the PHSs with giant planets are more depleted in refractory elements than those with rocky planets. Among the PHSs with rocky planets, the refractory depletion trends are potentially correlated with the terrestrial planets’ radii and multiplicity. In the cases of PHSs with giant planets, sub-Jovian PHSs demonstrate more depleted refractory trends than stars hosting Jovian-mass planets, raising questions on different planetary formation processes for Neptune-like and Jupiter-like planets.

 

Activity tracking has the potential to promote active lifestyles among older adults. However, current activity tracking technologies may inadvertently perpetuate ageism by focusing on age-related health risks. Advocating for a personalized approach in activity tracking technology, we sought to understand what activities older adults find meaningful to track and the underlying values of those activities. We conducted a reflective interview study following a 7-day activity journaling with 13 participants. We identified various underlying values motivating participants to track activities they deemed meaningful. These values, whether competing or aligned, shape the desirability of activities. Older adults appreciate low-exertion activities, but they are difficult to track. We discuss how these activities can become central in designing activity tracking systems. Our research offers insights for creating value-driven, personalized activity trackers that resonate more fully with the meaningful activities of older adults. 

Sepsis, whole‐body inflammation caused by the contamination of blood by bacteria and endotoxins, affects millions of patients annually with high mortality rates. A recent promising approach to treat sepsis involves the removal of bacteria and endotoxins using extracorporeal blood‐cleansing devices. However, poor specificity, slow recognition of pathogens, and high costs remain the main limitations. Here, the melanin, a biologically derived pigment, is reported for the rapid binding of bacteria and endotoxins from the contaminated blood . This novel approach utilizes the specific binding between Zn²⁺‐loaded melanin and bacteria/endotoxins with minimal nonspecific interactions with human blood components. Melanin contains various chemical functional groups that allow reversible chelation of metallic ions such as Zn²⁺via redox reactions. Zn²⁺enables rapid and specific binding with bacteria/endotoxins due to the strong electrostatic interactions between Zn²⁺and phosphate ions. The presence of various zinc‐binding proteins on the bacterial cell membrane further enhances the binding. The well‐known biocompatibility and low cost make melanin an ideal material to interface with human blood. Zn²⁺‐charged melanin can remove 90% ofE. coliand 100% of endotoxin in PBS and human blood. Zn²⁺‐melanin also demonstrated excellent hemocompatibility shown by protein adsorption, blood coagulation, and hemolysis tests.

 

Viruses impact microbial systems through killing hosts, horizontal gene transfer, and altering cellular metabolism, consequently impacting nutrient cycles. A virus-infected cell, a “virocell,” is distinct from its uninfected sister cell as the virus commandeers cellular machinery to produce viruses rather than replicate cells. Problematically, virocell responses to the nutrient-limited conditions that abound in nature are poorly understood. Here we used a systems biology approach to investigate virocell metabolic reprogramming under nutrient limitation. Using transcriptomics, proteomics, lipidomics, and endo- and exo-metabolomics, we assessed how low phosphate (low-P) conditions impacted virocells of a marine Pseudoalteromonas host when independently infected by two unrelated phages (HP1 and HS2). With the combined stresses of infection and nutrient limitation, a set of nested responses were observed. First, low-P imposed common cellular responses on all cells (virocells and uninfected cells), including activating the canonical P-stress response, and decreasing transcription, translation, and extracellular organic matter consumption. Second, low-P imposed infection-specific responses (for both virocells), including enhancing nitrogen assimilation and fatty acid degradation, and decreasing extracellular lipid relative abundance. Third, low-P suggested virocell-specific strategies. Specifically, HS2-virocells regulated gene expression by increasing transcription and ribosomal protein production, whereas HP1-virocells accumulated host proteins, decreased extracellular peptide relative abundance, and invested in broader energy and resource acquisition. These results suggest that although environmental conditions shape metabolism in common ways regardless of infection, virocell-specific strategies exist to support viral replication during nutrient limitation, and a framework now exists for identifying metabolic strategies of nutrient-limited virocells in nature.

 

We present a chemical and dynamical analysis of the leading arm (LA) and trailing arm (TA) of the Sagittarius (Sgr) stream, as well as for the Sgr dwarf galaxy core (SC), using red giant branch, main-sequence, and RR Lyrae stars from large spectroscopic survey data. The different chemical properties among the LA, TA, and SC generally agree with recent studies and can be understood by a radial metallicity gradient established in the progenitor of the Sgr dwarf, followed by preferential stellar stripping from the outer part of the Sgr progenitor. One striking finding is a relatively larger fraction of low-eccentricity stars (e< 0.4) in the LA than in the TA and SC. The TA and SC exhibit very similar distributions. Considering that a tidal tail stripped off from a dwarf galaxy maintains the orbital properties of its progenitor, we expect that thee-distribution of the LA should be similar to that of the TA and SC. Thus, the disparate behavior of thee-distribution of the LA is of particular interest. Following the analysis of Vasiliev et al., we attempt to explain the differente-distribution by introducing a time-dependent perturbation of the Milky Way by the Large Magellanic Cloud's (LMC) gravitational pull, resulting in substantial evolution of the angular momentum of the LA stars to produce the low-estars. In addition, we confirm from RR Lyrae stars with high eccentricity (e> 0.6) that the TA stars farther away from the SC are also affected by disturbances from the LMC.