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Creators/Authors contains: "Kukula, Kaitlyn"

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  1. Raman spectroscopy is a common identification and analysis technique used in research and manufacturing industries. This study investigates the use of Raman spectroscopy and deep learning techniques for identifying various nanofabrication chemicals. Four solvents and SU-8 developer were identified inside common chemical storage and distribution containers. The containers attenuated the spectra and contributed varying amounts of background fluorescence, making manual identification difficult. Two varieties of SU-8 photoresist were differentiated inside amber glass jars, and cured samples of three ratios of polydimethylsiloxane (PDMS) were differentiated using Raman microscopy. The neural network accurately identified the nanofabrication chemicals 100% of the time, without additional preprocessing. This investigation demonstrates the use of Raman spectroscopy and neural networks for the identification of nanofabrication chemicals and makes recommendations for use in other challenging identification applications. 
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  2. null (Ed.)
    Bacteria identification can be a time-consuming process. Machine learning algorithms that use deep convolutional neural networks (CNNs) provide a promising alternative. Here, we present a deep learning based approach paired with Raman spectroscopy to rapidly and accurately detect the identity of a bacteria class. We propose a simple 4-layer CNN architecture and use a 30-class bacteria isolate dataset for training and testing. We achieve an identification accuracy of around 86% with identification speeds close to real-time. This optical/biological detection method is promising for applications in the detection of microbes in liquid biopsies and concentrated environmental liquid samples, where fast and accurate detection is crucial. This study uses a recently published dataset of Raman spectra from bacteria samples and an improved CNN model built with TensorFlow. Results show improved identification accuracy and reduced network complexity. 
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