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Standard zebrafish transgenesis involves random transgene integration with resource-intensive screening. While phiC31 integrase–basedattP/attBrecombination has streamlined transgenesis in mice andDrosophila, validatedattP-based landing sites for universal applications are lacking in zebrafish. Here, we developedphiC31 Integrase Genomic Loci Engineered for Transgenesis(pIGLET) as transgenesis approach, with twoattPlanding sitespIGLET14aandpIGLET24bfrom well-validated Tol2 transgenes. Both sites facilitate diverse transgenesis applications including reporters and Cre/loxPtransgenes. ThepIGLET14aandpIGLET24blanding sites consistently yield 25 to 50% germline transmission, substantially reducing the resources needed for transgenic line generation. Transgenesis into these sites enables reproducible expression patterns in F0 zebrafish embryos for enhancer discovery and testing of gene regulatory variants. Together, our new landing sites streamline targeted, reproducible zebrafish transgenesis as a robust platform for various applications while minimizing the workload for generating transgenic lines.