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Free, publicly-accessible full text available March 18, 2023
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Aims. An interplanetary coronal mass ejection (ICME) event was observed by the Solar Orbiter at 0.8 AU on 2020 April 19 and by Wind at 1 AU on 2020 April 20. Futhermore, an interplanetary shock wave was driven in front of the ICME. Here, we focus on the transmission of the magnetic fluctuations across the shock and we analyze the characteristic wave modes of solar wind turbulence in the vicinity of the shock observed by both spacecraft. Methods. The observed ICME event is characterized by a magnetic helicity-based technique. The ICME-driven shock normal was determined by magnetic coplanarity method formore »Free, publicly-accessible full text available December 1, 2022
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DenseNets introduce concatenation-type skip connections that achieve state-of-the-art accuracy in several computer vision tasks. In this paper, we reveal that the topology of the concatenation-type skip connections is closely related to the gradient propagation which, in turn, enables a predictable behavior of DNNs’ test performance. To this end, we introduce a new metric called NN-Mass to quantify how effectively information flows through DNNs. Moreover, we empirically show that NN-Mass also works for other types of skip connections, e.g., for ResNets, Wide-ResNets (WRNs), and MobileNets, which contain addition-type skip connections (i.e., residuals or inverted residuals). As such, for both DenseNet-like CNNsmore »
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Free, publicly-accessible full text available November 1, 2022
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Jacob, S. (Ed.)Protein–protein interactions underlie cellular structure and function. In recent years, a number of methods have been developed for the identification of protein complexes and component proteins involved in the control of various biological pathways. Tandem affinity purification (TAP) coupled with mass spectrometry (MS) is a powerful method enabling the isolation of high-purity native protein complexes under mild conditions by performing two sequential purification steps using two different epitope tags. In this protocol, we describe a TAP-MS methodology for identifying protein-protein interactions present at very low levels in the fungal cell. Using the 6xHis-3xFLAG double tag, we start the affinity purificationmore »
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Eukaryotic filamentous plant pathogens with biotrophic growth stages like the devastating hemibiotrophic rice blast fungus Magnaporthe oryzae grow for extended periods in living host plant cells without eliciting defense responses. M. oryzae elaborates invasive hyphae (IH) that grow in and between living rice cells while separated from host cytoplasm by plant-derived membrane interfaces. However, although critical to the plant infection process, the molecular mechanisms and metabolic strategies underpinning this intracellular growth phase are poorly understood. Eukaryotic cell growth depends on activated target-of-rapamycin (TOR) kinase signaling, which inhibits autophagy. Here, using live-cell imaging coupled with plate growth tests and RNAseq, proteomic,more »