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Hybridization probes have been used in the detection of specific nucleic acids for the last 50 years. Despite the extensive efforts and the great significance, the challenges of the commonly used probes include (1) low selectivity in detecting single nucleotide variations (SNV) at low ( e.g. room or 37 °C) temperatures; (2) low affinity in binding folded nucleic acids, and (3) the cost of fluorescent probes. Here we introduce a multicomponent hybridization probe, called OWL2 sensor, which addresses all three issues. The OWL2 sensor uses two analyte binding arms to tightly bind and unwind folded analytes, and two sequence-specific strands that bind both the analyte and a universal molecular beacon (UMB) probe to form fluorescent ‘OWL’ structure. The OWL2 sensor was able to differentiate single base mismatches in folded analytes in the temperature range of 5–38 °C. The design is cost-efficient since the same UMB probe can be used for detecting any analyte sequence.more » « less
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Fiumara, James ; Cieri, Christopher ; Wright, Jonathan ; Liberman, Mark ( , Proceedings of the LREC 2020 Workshop Citizen Linguistics in Language Resource Development (CLLRD 2020))This paper introduces the citizen science platform, LanguageARC, developed within the NIEUW (Novel Incentives and Workflows) project supported by the National Science Foundation under Grant No. 1730377. LanguageARC is a community- oriented online platform bringing together researchers and “citizen linguists” with the shared goal of contributing to linguistic research and language technology development. Like other Citizen Science platforms and projects, LanguageARC harnesses the power and efforts of volunteers who are motivated by the incentives of contributing to science, learning and discovery, and belonging to a community dedicated to social improvement. Citizen linguists contribute language data and judgments by participating in research tasks such as classifying regional accents from audio clips, recording audio of picture descriptions and answering personality questionnaires to create baseline data for NLP research into autism and neurodegenerative conditions. Researchers can create projects on Language ARC without any coding or HTML required using our Project Builder Toolkit.more » « less
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Fiumara, James ; Cieri, Christopher ; Liberman, Mark ; Callison-Burch, Chris ( , Proceedings of the Workshop on Citizen Linguistics in Language Resource Development (CLLRD 2020))