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  1. Abstract

    The TTG2 transcription factor ofArabidopsisregulates a set of epidermal traits, including the differentiation of leaf trichomes, flavonoid pigment production in cells of the inner testa (or seed coat) layer and mucilage production in specialized cells of the outer testa layer. Despite the fact that TTG2 has been known for over twenty years as an important regulator of multiple developmental pathways, little has been discovered about the downstream mechanisms by which TTG2 co-regulates these epidermal features. In this study, we present evidence of phosphoinositide lipid signaling as a mechanism for the regulation of TTG2-dependent epidermal pathways. Overexpression of theAtPLC1gene rescues the trichome and seed coat phenotypes of thettg2-1mutant plant. Moreover, in the case of seed coat color rescue,AtPLC1overexpression restored expression of the TTG2 flavonoid pathway target genes,TT12andTT13/AHA10. Consistent with these observations, a dominantAtPLC1T-DNA insertion allele (plc1-1D)promotes trichome development in both wild-type andttg2-3plants. Also,AtPLC1promoter:GUS analysis shows expression in trichomes and this expression appears dependent on TTG2. Taken together, the discovery of a genetic interaction betweenTTG2andAtPLC1suggests a role for phosphoinositide signaling in the regulation of trichome development, flavonoid pigment biosynthesis and the differentiation of mucilage-producing cells of the seed coat. This finding provides new avenues for future research at the intersection of the TTG2-dependent developmental pathways and the numerous molecular and cellular phenomena influenced by phospholipid signaling.

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