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  1. Free, publicly-accessible full text available June 1, 2023
  2. Free, publicly-accessible full text available December 22, 2022
  3. Abstract

    The differentiation of human induced pluripotent stem cells (hiPSCs) to prescribed cell fates enables the engineering of patient-specific tissue types, such as hyaline cartilage, for applications in regenerative medicine, disease modeling, and drug screening. In many cases, however, these differentiation approaches are poorly controlled and generate heterogeneous cell populations. Here, we demonstrate cartilaginous matrix production in three unique hiPSC lines using a robust and reproducible differentiation protocol. To purify chondroprogenitors (CPs) produced by this protocol, we engineered a COL2A1-GFP knock-in reporter hiPSC line by CRISPR-Cas9 genome editing. Purified CPs demonstrated an improved chondrogenic capacity compared with unselected populations. The ability to enrich for CPs and generate homogenous matrix without contaminating cell types will be essential for regenerative and disease modeling applications. Stem Cells  2019;37:65–76

    </sec></div> <div class="actions" style="padding-left:10px;"> <span class="reader-count"> <a class="misc doi-link" href="https://doi.org/10.1002/stem.2931" target="_blank" title="Link to document DOI" data-ostiid="10363227"> Full Text Available <span class="fas fa-external-link-alt"></span> </a> </span> </div> </div><div class="clearfix"></div> </div> </li> <li> <div class="article item document" itemscope itemtype="http://schema.org/TechArticle"> <div class="item-info"> <div class="title"> <a href="https://par.nsf.gov/biblio/10005883-separated-response-functions-exclusive-forward-electroproduction-deuterium" itemprop="url"> <span itemprop="name">Separated response functions in exclusive, forward <math><msup><mrow><mi>π</mi></mrow><mo>±</mo></msup></math> electroproduction on deuterium</span> </a> </div> <div> <strong> <a class="misc doi-link" href="https://doi.org/10.1103/PhysRevC.91.015202" target="_blank" title="Link to document DOI">https://doi.org/10.1103/PhysRevC.91.015202  <span class="fas fa-external-link-alt"></span></a> </strong> </div> <div class="metadata"> <span class="authors"> <span class="author" itemprop="author">Huber, G. M.</span> <span class="sep">; </span><span class="author" itemprop="author">Blok, H. P.</span> <span class="sep">; </span><span class="author" itemprop="author">Butuceanu, C.</span> <span class="sep">; </span><span class="author" itemprop="author">Gaskell, D.</span> <span class="sep">; </span><span class="author" itemprop="author">Horn, T.</span> <span class="sep">; </span><span class="author" itemprop="author">Mack, D. J.</span> <span class="sep">; </span><span class="author" itemprop="author">Abbott, D.</span> <span class="sep">; </span><span class="author" itemprop="author">Aniol, K.</span> <span class="sep">; </span><span class="author" itemprop="author">Anklin, H.</span> <span class="sep">; </span><span class="author" itemprop="author">Armstrong, C.</span> <span class="sep">; </span><span class="author">et al</span></span> <span class="year">( <time itemprop="datePublished" datetime="2015-01-01">January 2015</time> , Physical Review C) </span> </div> <div class="actions" style="padding-left:10px;"> <span class="reader-count"> <a class="misc doi-link" href="https://doi.org/10.1103/PhysRevC.91.015202" target="_blank" title="Link to document DOI" data-ostiid="10005883"> Full Text Available <span class="fas fa-external-link-alt"></span> </a> </span> </div> </div><div class="clearfix"></div> </div> </li> <li> <div class="article item document" itemscope itemtype="http://schema.org/TechArticle"> <div class="item-info"> <div class="title"> <a href="https://par.nsf.gov/biblio/10003387-measurement-parity-violating-asymmetry-inclusive-electroproduction-near-resonance" itemprop="url"> <span itemprop="name">Measurement of the Parity-Violating Asymmetry in Inclusive Electroproduction of <math display='inline'><msup><mi>π</mi><mo>−</mo></msup></math> near the <math display='inline'><msup><mi>Δ</mi><mn>0</mn></msup></math> Resonance</span> </a> </div> <div> <strong> <a class="misc doi-link" href="https://doi.org/10.1103/PhysRevLett.108.122002" target="_blank" title="Link to document DOI">https://doi.org/10.1103/PhysRevLett.108.122002  <span class="fas fa-external-link-alt"></span></a> </strong> </div> <div class="metadata"> <span class="authors"> <span class="author" itemprop="author">Androić, D.</span> <span class="sep">; </span><span class="author" itemprop="author">Armstrong, D. S.</span> <span class="sep">; </span><span class="author" itemprop="author">Arvieux, J.</span> <span class="sep">; </span><span class="author" itemprop="author">Bailey, S. L.</span> <span class="sep">; </span><span class="author" itemprop="author">Beck, D. H.</span> <span class="sep">; </span><span class="author" itemprop="author">Beise, E. J.</span> <span class="sep">; </span><span class="author" itemprop="author">Benesch, J.</span> <span class="sep">; </span><span class="author" itemprop="author">Benmokhtar, F.</span> <span class="sep">; </span><span class="author" itemprop="author">Bimbot, L.</span> <span class="sep">; </span><span class="author" itemprop="author">Birchall, J.</span> <span class="sep">; </span><span class="author">et al</span></span> <span class="year">( <time itemprop="datePublished" datetime="2012-03-01">March 2012</time> , Physical Review Letters) </span> </div> <div class="actions" style="padding-left:10px;"> <span class="reader-count"> <a class="misc doi-link" href="https://doi.org/10.1103/PhysRevLett.108.122002" target="_blank" title="Link to document DOI" data-ostiid="10003387"> Full Text Available <span class="fas fa-external-link-alt"></span> </a> </span> </div> </div><div class="clearfix"></div> </div> </li> <li> <div class="article item document" itemscope itemtype="http://schema.org/TechArticle"> <div class="item-info"> <div class="title"> <a href="https://par.nsf.gov/biblio/10003250-new-measurements-high-momentum-nucleons-short-range-structures-nuclei" itemprop="url"> <span itemprop="name">New Measurements of High-Momentum Nucleons and Short-Range Structures in Nuclei</span> </a> </div> <div> <strong> <a class="misc doi-link" href="https://doi.org/10.1103/PhysRevLett.108.092502" target="_blank" title="Link to document DOI">https://doi.org/10.1103/PhysRevLett.108.092502  <span class="fas fa-external-link-alt"></span></a> </strong> </div> <div class="metadata"> <span class="authors"> <span class="author" itemprop="author">Fomin, N.</span> <span class="sep">; </span><span class="author" itemprop="author">Arrington, J.</span> <span class="sep">; </span><span class="author" itemprop="author">Asaturyan, R.</span> <span class="sep">; </span><span class="author" itemprop="author">Benmokhtar, F.</span> <span class="sep">; </span><span class="author" itemprop="author">Boeglin, W.</span> <span class="sep">; </span><span class="author" itemprop="author">Bosted, P.</span> <span class="sep">; </span><span class="author" itemprop="author">Bruell, A.</span> <span class="sep">; </span><span class="author" itemprop="author">Bukhari, M. H.</span> <span class="sep">; </span><span class="author" itemprop="author">Christy, M. E.</span> <span class="sep">; </span><span class="author" itemprop="author">Chudakov, E.</span> <span class="sep">; </span><span class="author">et al</span></span> <span class="year">( <time itemprop="datePublished" datetime="2012-02-01">February 2012</time> , Physical Review Letters) </span> </div> <div class="actions" style="padding-left:10px;"> <span class="reader-count"> <a class="misc doi-link" href="https://doi.org/10.1103/PhysRevLett.108.092502" target="_blank" title="Link to document DOI" data-ostiid="10003250"> Full Text Available <span class="fas fa-external-link-alt"></span> </a> </span> </div> </div><div class="clearfix"></div> </div> </li> <li> <div class="article item document" itemscope itemtype="http://schema.org/TechArticle"> <div class="item-info"> <div class="title"> <a href="https://par.nsf.gov/biblio/10011128-transverse-beam-spin-asymmetries-backward-angles-elastic-electron-proton-quasielastic-electron-deuteron-scattering" itemprop="url"> <span itemprop="name">Transverse Beam Spin Asymmetries at Backward Angles in Elastic Electron-Proton and Quasielastic Electron-Deuteron Scattering</span> </a> </div> <div> <strong> <a class="misc doi-link" href="https://doi.org/10.1103/PhysRevLett.107.022501" target="_blank" title="Link to document DOI">https://doi.org/10.1103/PhysRevLett.107.022501  <span class="fas fa-external-link-alt"></span></a> </strong> </div> <div class="metadata"> <span class="authors"> <span class="author" itemprop="author">Androić, D.</span> <span class="sep">; </span><span class="author" itemprop="author">Armstrong, D. S.</span> <span class="sep">; </span><span class="author" itemprop="author">Arvieux, J.</span> <span class="sep">; </span><span class="author" itemprop="author">Bailey, S. L.</span> <span class="sep">; </span><span class="author" itemprop="author">Beck, D. H.</span> <span class="sep">; </span><span class="author" itemprop="author">Beise, E. J.</span> <span class="sep">; </span><span class="author" itemprop="author">Benesch, J.</span> <span class="sep">; </span><span class="author" itemprop="author">Benmokhtar, F.</span> <span class="sep">; </span><span class="author" itemprop="author">Bimbot, L.</span> <span class="sep">; </span><span class="author" itemprop="author">Birchall, J.</span> <span class="sep">; </span><span class="author">et al</span></span> <span class="year">( <time itemprop="datePublished" datetime="2011-07-01">July 2011</time> , Physical Review Letters) </span> </div> <div class="actions" style="padding-left:10px;"> <span class="reader-count"> <a class="misc doi-link" href="https://doi.org/10.1103/PhysRevLett.107.022501" target="_blank" title="Link to document DOI" data-ostiid="10011128"> Full Text Available <span class="fas fa-external-link-alt"></span> </a> </span> </div> </div><div class="clearfix"></div> </div> </li> </ol> <div id="pagination-lower" style="display:none;"> <div class="pull-right" style="line-height: 30px;"> <div class="btn-group pagination nomargin"> <a href="#" class="btn btn-sm btn-default noborderradius" disabled="disabled">«<span class="hidden-xs"> Prev</span></a> <a class="dropdown-toggle btn btn-sm btn-default paging-dropdown disabled hidden-xs noborderradius" href="#" data-toggle="dropdown"> <span class="caret"></span> <span class="sr-only">Select page number</span> </a> <a href="#" class="btn btn-sm btn-default noborderradius" disabled="disabled"><span class="hidden-xs">Next </span>»</a> </div> </div> <div class="clearfix"></div> </div> </div> </div> </div> <!-- / basic search results --> </div> </div> <input type='hidden' id='schtype' value=''/> </div> </div> <footer class="row" id="footer-wrapper"> <div class="footer-content"> <div id="footerOSTI" class=" hidden-print"> <ul> <li><a target="_blank" rel="noreferrer" href="http://www.nsf.gov/policies/">Website Policies</a> | <a target="_blank" rel="noreferrer" href="http://www.nsf.gov/about/performance/">Budget and Performance</a> | <a target="_blank" rel="noreferrer" href="http://www.nsf.gov/oig/">Inspector General</a> | <a target="_blank" rel="noreferrer" href="http://www.nsf.gov/policies/privacy.jsp">Privacy</a> | <a target="_blank" rel="noreferrer" href="http://www.nsf.gov/policies/foia.jsp">FOIA</a> | <a target="_blank" rel="noreferrer" href="http://www.nsf.gov/od/odi/notice.jsp">No FEAR Act</a> | <a target="_blank" rel="noreferrer" href="http://usa.gov">USA.gov</a> | <a target="_blank" rel="noreferrer" href="http://www.nsf.gov/policies/access.jsp">Accessibility</a> | <a target="_blank" rel="noreferrer" href="http://www.nsf.gov/policies/nsf_plain_language.jsp">Plain Language</a> | <a target="_blank" rel="noreferrer" href="http://www.nsf.gov/help/contact.jsp">Contact</a> | <a target="_blank" rel="noreferrer" href="https://nsf.gov/help/">Help</a> </li> </ul> The National Science Foundation, 2415 Eisenhower Avenue, Alexandria, Virginia 22314, USA Tel: (703) 292-5111, FIRS: (800) 877-8339 | TDD: (800) 281-8749 </div> </div> </footer> </div> <div id="authorselect" class="modal" tabindex="-1" role="dialog" aria-labelledby="authorselect_label" aria-hidden="true"> <div class="modal-dialog"> <div class="modal-content"> <div class="modal-header"> <button type="button" class="close" data-dismiss="modal" aria-hidden="true">×</button> <div id="authorselect_label">Author Select</div> </div> <form id="authorselect-form" style="margin-bottom: 0px;"> <input type="hidden" name="pg" id="authorselect-pg" value="1" /> <div class="modal-body" id="authorselect_body"> <div class="row"> <div class="col-md-4"> <label for="authorselect-lname">Last Name:</label><br /> <input type="text" name="lname" class="input-sm form-control" id="authorselect-lname" placeholder="Last name" /><br /> </div> <div class="col-md-4"> <label for="authorselect-fname">First Name:</label><br /> <input type="text" name="fname" class="input-sm form-control" id="authorselect-fname" placeholder="First name" /> </div> <div class="col-md-2">  <br /> <a href="#" onclick="$('#authorselect-form').submit(); return false;" class="btn btn-sm btn-default"><span class="fas fa-search"></span><span class="sr-only">Search</span></a> </div> </div> <div class="push_top"></div> <div class="row"> <div class="col-md-12"> <ul class="nav nav-tabs"> <li class="active"><a href="#authorselect-tab-res" id="authorselect-tab-res-btn" data-toggle="tab">Search Results</a></li> <li><a href="#authorselect-tab-sel" id="authorselect-tab-sel-btn" data-toggle="tab">Selected Authors</a></li> </ul> <div class="tab-content"> <div class="tab-pane active" id="authorselect-tab-res" style="max-height: 450px;"> <div class="padding text-muted" id="authorselect-tab-res-content">Type in a name, or the first few letters of a name, in one or both of appropriate search boxes above and select the search button. 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