skip to main content

Explore Research Products in the PAR
It may take a few hours for recently added research products to appear in PAR search results.


  • Home
  • Search Results
  • Page 1 of 1

Search for: All records

Creators/Authors contains: "Markossian, Sarine"

Note: When clicking on a Digital Object Identifier (DOI) number, you will be taken to an external site maintained by the publisher. Some full text articles may not yet be available without a charge during the embargo (administrative interval).
What is a DOI Number?

Some links on this page may take you to non-federal websites. Their policies may differ from this site.

  1. ; ; ; ; ; ( , Current Protocols in Chemical Biology)
    Abstract

    Drug‐induced liver injury is an important cause of non‐approval in drug development and the withdrawal of already approved drugs from the market. Screening human hepatic cell lines for toxicity has been used extensively to predict drug‐induced liver injury in preclinical drug development. Assessing hepatic‐cell health with more diverse markers will increase the value of in vitro assays and help predict the mechanism of toxicity. We describe three live cell‐based assays using HepG2 cells to measure cell health parameters indicative of hepatotoxicity. The first assay measures cellular ATP levels using luciferase. The second and third assays are multiparametric high‐content screens covering a panel of cell health markers including cell count, mitochondrial membrane potential and structure, nuclear morphology, vacuolar density, and reactive oxygen species and glutathione levels. © 2020 Wiley Periodicals LLC.

    Basic Protocol 1: Measurement of cellular ATP content

    Basic Protocol 2: High‐content analysis assay to assess cell count, mitochondrial membrane potential and structure, and reactive oxygen species

    Basic Protocol 3: High‐content analysis assay to assess nuclear morphology, vacuoles, and glutathione content

    Support Protocol 1: Subculturing and maintaining HepG2 cells

    Support Protocol 2: Plating HepG2 cell line

    Support Protocol 3: Transferring compounds by pin tool

    Support Protocol 4: Generating dose‐response curves

     
    more » « less