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  1. ABSTRACT Using image analysis of scanning electron micrographs (SEMs), we compared differences in growth of D-stage veligers [i.e. prodissoconch I and II (PI and PII) larvae] of eastern oysters Crassostrea virginica grown in mesohaline water under high- and low-CO2 conditions. We found SEMs to reveal no evidence of dissolution or shell structure deformity for larval shells in either of the CO2 treatments but detected prominent growth lines in the PII regions of larval shells. The number of growth lines closely approximated the duration of the experiment, suggesting that growth lines are generated daily. Mean growth line interval widths were 20%more »greater for larval shells cultured in low- vs high-CO2 conditions. Crassostrea virginica veliger larvae were shown to tolerate high CO2 levels and aragonite saturation states (Ωarag) < 1.0, but larval growth was slowed substantially under these conditions. Differences in growth line interval width translate into substantial changes in shell area and account for previously observed differences in total shell area between the treatments, as determined by light microscopy and image analysis. Other studies have documented high mortality and malformation of D-stage larvae in bivalves when pre-veliger life stages (i.e. eggs, gastrula and trochophores) were exposed to elevated CO2. Our experiments revealed statistical differences in rates of larval survival, settlement and subsequent early-stage spat mortality for veligers reared in high- and low-CO2 conditions. Although each of these rates was measurably affected by high CO2, the magnitude of these differences was small (range across categories = 0.7–6.3%) suggesting that the impacts may not be catastrophic, as implied by several previous studies. We believe the apparent disparity among experimental results may be best explained by differential vulnerability of pre-veliger stage larvae and veligers, whereby PI and PII larvae have greater physiological capacity to withstand environmental conditions that may be thermodynamically unfavourable to calcification (i.e. Ωarag < 1.0).« less
  2. We present 42 rapidly evolving (time spent above half-maximum brightness t1/2<12d) extragalactic transients from Phase I of the Zwicky Transient Facility (ZTF), of which 22 have spectroscopic classifications. This is one of the largest systematically selected samples of day-timescale transients, and the first with spectroscopic classifications. Most can be classified as core-collapse supernovae (SNe), and we identify several predominant subtypes: (1) subluminous Type IIb or Type Ib SNe; (2) luminous Type Ibn or hybrid IIn/Ibn SNe; and (3) radio-loud, short-duration luminous events similar to AT2018cow. We conclude that rates quoted in the literature for rapidly evolving extragalactic transients are dominatedmore »by the subluminous events (mostly Type IIb SNe). From our spectroscopic classifications and radio, X-ray, and millimeter-band upper limits, we are motivated to consider the AT2018cow-like objects a distinct class, and use ZTF's systematic classification experiments to calculate that their rate does not exceed 0.1% of the local core-collapse SN rate, in agreement with previous work. By contrast, most other events are simply the extreme of a continuum of established SN types extending to ordinary timescales. The light curves of our objects are very similar to those of unclassified events in the literature, illustrating how spectroscopically classified samples of low-redshift objects in shallow surveys like ZTF can be used to photometrically classify larger numbers of events at higher redshift.« less
    Free, publicly-accessible full text available May 18, 2022
  3. Free, publicly-accessible full text available May 1, 2022
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  5. Free, publicly-accessible full text available August 16, 2022
  6. Recently, a variety of enzymes have been found to accept electrons from NAD(P)H yet reduce lower-potential carriers such as ferredoxin and flavodoxin semiquinone, in apparent violation of thermodynamics. The reaction is favorable overall, however, because these enzymes couple the foregoing endergonic one-electron transfer to exergonic transfer of the other electron from each NAD(P)H, in a process called 'flavin-based electron bifurcation'. The reduction midpoint potentials (E°s) of the multiple flavins in these enzymes are critical to their mechanisms. We describe methods we have found to be useful for measuring each of the E°s of each of the flavins in bifurcating electronmore »transfer flavoproteins.« less
  7. Gravitational waves provide a unique tool for observational astronomy. While the first LIGO–Virgo catalogue of gravitational wave transients (GWTC-1) contains 11 signals from black hole and neutron star binaries, the number of observations is increasing rapidly as detector sensitivity improves. To extract information from the observed signals, it is imperative to have fast, flexible, and scalable inference techniques. In a previous paper, we introduced BILBY: a modular and user-friendly Bayesian inference library adapted to address the needs of gravitational-wave inference. In this work, we demonstrate that BILBY produces reliable results for simulated gravitational-wave signals from compact binary mergers, and verifymore »that it accurately reproduces results reported for the 11 GWTC-1 signals. Additionally, we provide configuration and output files for all analyses to allow for easy reproduction, modification, and future use. This work establishes that BILBY is primed and ready to analyse the rapidly growing population of compact binary coalescence gravitational-wave signals.« less