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Abstract This study analyzes observations collected by multilevel towers to estimate turbulence parameters in the atmospheric surface layer of two landfalling tropical cyclones (TCs). The momentum flux, turbulent kinetic energy (TKE) and dissipation rate increase with the wind speed independent of surface types. However, the momentum flux and TKE are much larger over land than over the coastal ocean at a given wind speed range. The vertical eddy diffusivity is directly estimated using the momentum flux and strain rate, which more quickly increases with the wind speed over a rougher surface. Comparisons of the eddy diffusivity estimated using the direct flux method and that using the friction velocity and height show good agreement. On the other hand, the traditional TKE method overestimates the eddy diffusivity compared to the direct flux method. The scaling coefficients in the TKE method are derived for the two different surface types to better match with the vertical eddy diffusivity based on the direct flux method. Some guidance to improve vertical diffusion parameterizations for TC landfall forecasts in weather simulations are also provided.