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Abstract This study examines climatological potential vorticity streamer (PVS) activity associated with Rossby wave breaking (RWB), which can impact TC activity in the subtropical North Atlantic (NATL) basin via moisture and wind anomalies. PVSs are identified along the 2-PVU (1 PVU = 10 −6 K kg −1 m 2 s −1 ) contour on the 350-K isentropic surface, using a unique identification technique that combines previous methods. In total, 21 149 individual PVS instances are identified from the ERA-Interim (ERAI) climatology during June–November over 1979–2015 with a peak in July–August. The total number of PVSs identified in this study is more than previous PVS climatologies for this region, since the new technique identifies a wider range of cases. Variations in PVS size and intensity prompt the development of a new PVS activity index (PVSI), which provides an integrated measure of PVS activity that can improve comparisons with TC activity. For instance, PVSI has a stronger negative correlation with seasonal TC activity ( r = −0.55) relative to PVS frequency, size, or intensity alone. PVSI in June–July is also positively correlated with PVSI in August–November ( r = 0.67), suggesting predictive capability. Compared to the ERAI and Japan Meteorological Agency 55-Year Reanalysis (JRA-55) climatology, there are more PVSs in the Climate Forecast System Reanalysis (CFSR) but these have weaker average intensity overall. While no long-term trend in PVSI is observed in the ERAI or JRA-55 climatologies, a negative trend is observed in CFSR, which could be related to differences in near tropopause static stability early in the climatological period (1979–86) between the CFSR and ERAI datasets.