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Heparin is an essential anticoagulant used for treating and preventing thrombosis. However, the complexity of heparin has hindered the development of a recombinant source, making its supply dependent on a vulnerable animal population. In nature, heparin is produced exclusively in mast cells, which are not suitable for commercial production, but mastocytoma cells are readily grown in culture and make heparan sulfate, a closely related glycosaminoglycan that lacks anticoagulant activity. Using gene expression profiling of mast cells as a guide, a multiplex genome engineering strategy was devised to produce heparan sulfate with high anticoagulant potency and to eliminate contaminating chondroitin sulfatemore »Free, publicly-accessible full text available January 1, 2023
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In engineering, students’ completion of prerequisites indicates an understanding of fundamental knowledge. Recent studies have shown a significant relationship between student performance and prior knowledge. Weak knowledge retention from prerequisite coursework can present challenges in progressive learning. This study investigates the relationship between prior knowledge and students’ performance over a few courses of Statics. Statistics has been considered as the subject of interest since it is the introductory engineering course upon which many subsequent engineering courses rely, including many engineering analysis and design courses. The prior knowledge was determined based on the quantitative and qualitative preparedness. A quiz set wasmore »Free, publicly-accessible full text available July 26, 2022
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Free, publicly-accessible full text available November 1, 2022
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Free, publicly-accessible full text available December 1, 2022
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Monitoring water quality by detecting chemical and biological contaminants is critical to ensuring the provision and discharge of clean water, hence protecting human health and the ecosystem. Among the available analytical techniques, infrared (IR) spectroscopy provides sensitive and selective detection of multiple water contaminants. In this work, we present an application of IR spectroscopy for qualitative and quantitative assessment of chemical and biological water contaminants. We focus on in-line detection of nitrogen pollutants in the form of nitrate and ammonium for wastewater treatment process control and automation. We discuss the effects of water quality parameters such as salinity, pH, andmore »
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Free, publicly-accessible full text available August 1, 2022
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Abstract The intensity of deep convective storms is driven in part by the strength of their updrafts and cold pools. In spite of the importance of these storm features, they can be poorly represented within numerical models. This has been attributed to model parameterizations, grid resolution, and the lack of appropriate observations with which to evaluate such simulations. The overarching goal of the Colorado State University Convective CLoud Outflows and UpDrafts Experiment (C 3 LOUD-Ex) was to enhance our understanding of deep convective storm processes and their representation within numerical models. To address this goal, a field campaign was conductedmore »