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Online search and brokering (OSB) is the phenomenon in which youth search for information for their immigrant families on the Internet. Few studies exist on reflecting on the past as youth conduct OSB from childhood to adolescence to young adulthood. We conducted a retrospective study using semi-structured interviews of 27 young adults (ages 19 - 24) to examine their experience and perceptions of OSB over time (primary schooling, secondary schooling, undergraduate). We found that although all the interviewees shared a common experience of being an online searcher and information broker for their immigrant families, their stories exist on various dimensions which are constantly shifting and changing over time, as ecological factors influence searching behaviors. We discuss how the results from our study can serve as future references for researchers doing work in search research and designers who are currently working on improving design tools for searching. 

Multidrug resistance continues to be a barrier to the effectiveness of highly active antiretroviral therapy in the treatment of human immunodeficiency virus 1 (HIV-1) infection. Darunavir (DRV) is a highly potent protease inhibitor (PI) that is oftentimes effective when drug resistance has emerged against first-generation inhibitors. Resistance to darunavir does evolve and requires 10–20 amino acid substitutions. The conformational landscapes of six highly characterized HIV-1 protease (PR) constructs that harbor up to 19 DRV-associated mutations were characterized by distance measurements with pulsed electron double resonance (PELDOR) paramagnetic resonance spectroscopy, namely double electron–electron resonance (DEER). The results show that the accumulated substitutions alter the conformational landscape compared to PI-naïve protease where the semi-open conformation is destabilized as the dominant population with open-like states becoming prevalent in many cases. A linear correlation is found between values of the DRV inhibition parameter Ki and the open-like to closed-state population ratio determined from DEER. The nearly 50% decrease in occupancy of the semi-open conformation is associated with reduced enzymatic activity, characterized previously in the literature.