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This study investigates variability of the South Pacific western subtropical mode water (SPWSTMW), its physical processes, and relationship with El Niño‐Southern Oscillation (ENSO), using a gridded Argo data product from January 2004 to September 2019. On seasonal timescale, the SPWSTMW volume shows a significant variability, which involves three periods: the formation period (June–October), the isolation period (November–February), and the dissipation period (March–May). This seasonal variability is related to seasonal fluctuation of the mixed layer depth. During the Argo period from 2004 to 2019, interannual variability of the SPWSTMW volume is tightly linked to the ENSO, increasing during El Niño periods and decreasing during La Niña periods. Further analyses indicate that ENSO‐related anomalous winds are primarily responsible for interannual variability of the SPWSTMW volume. The anomalous winds first influence the surface heat flux through evaporation and then the mixed layer depth through convection, leaving an imprint of ENSO on the SPWSTMW. This study also shows that the SPWSTMW responds differently to the central Pacific (CP) El Niño and eastern Pacific (EP) El Niño.