When trialkylamines are added to buffered solutions of peptides, unexpected adducts can be formed. These adducts correspond to Schiff base products. The source of the reaction is the unexpected presence of aldehydes in amines. The aldehydes can be detected in a few ways. Most importantly, they can lead to unanticipated results in proteomics experiments. Their undesirable effects can be minimized through the addition of other amines.
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Anil Sushma, Arathi ; Zhao, Bingqing ; Tsvetkova, Irina B. ; Pérez-Segura, Carolina ; Hadden-Perilla, Jodi A. ; Reilly, James P. ; Dragnea, Bogdan ( , The Journal of Physical Chemistry B)
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Zhao, Bingqing ; Reilly, Colin P. ; Davis, Caroline ; Matouschek, Andreas ; Reilly, James P. ( , Journal of Proteome Research)
