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ABSTRACT The contractile vacuole complex (CVC) is a dynamic and morphologically complex membrane organelle, comprising a large vesicle (bladder) linked with a tubular reticulum (spongiome). CVCs provide key osmoregulatory roles across diverse eukaryotic lineages, but probing the mechanisms underlying their structure and function is hampered by the limited tools available for in vivo analysis. In the experimentally tractable ciliate Tetrahymena thermophila, we describe four proteins that, as endogenously tagged constructs, localize specifically to distinct CVC zones. The DOPEY homolog Dop1p and the CORVET subunit Vps8Dp localize both to the bladder and spongiome but with different local distributions that are sensitive to osmotic perturbation, whereas the lipid scramblase Scr7p colocalizes with Vps8Dp. The H+-ATPase subunit Vma4 is spongiome specific. The live imaging permitted by these probes revealed dynamics at multiple scales including rapid exchange of CVC-localized and soluble protein pools versus lateral diffusion in the spongiome, spongiome extension and branching, and CVC formation during mitosis. Although the association with DOP1 and VPS8D implicate the CVC in endosomal trafficking, both the bladder and spongiome might be isolated from bulk endocytic input. 

Individuals experiencing unexpected distressing events, shocks, often rely on their social network for support. While prior work has shown how social networks respond to shocks, these studies usually treat all ties equally, despite differences in the support provided by different social relationships. Here, we conduct a computational analysis on Twitter that examines how responses to online shocks differ by the relationship type of a user dyad. We introduce a new dataset of over 13K instances of individuals' self-reporting shock events on Twitter and construct networks of relationship-labeled dyadic interactions around these events. By examining behaviors across 110K replies to shocked users in a pseudo-causal analysis, we demonstrate relationship-specific patterns in response levels and topic shifts. We also show that while well-established social dimensions of closeness such as tie strength and structural embeddedness contribute to shock responsiveness, the degree of impact is highly dependent on relationship and shock types. Our findings indicate that social relationships contain highly distinctive characteristics in network interactions, and that relationship-specific behaviors in online shock responses are unique from those of offline settings. 

The public interest in accurate scientific communication, underscored by recent public health crises, highlights how content often loses critical pieces of information as it spreads on-line. However, multi-platform analyses of this phenomenon remain limited due to challenges in data collection. Collecting mentions of research tracked by Altmetric LLC, we examine information retention in the over 4 million online posts referencing 9,765 of the most-mentioned scientific articles across blog sites, Facebook, news sites, Twitter, and Wikipedia. To do so, we present a burst-based framework for examining online discussions about science over time and across different platforms. To measure information retention, we develop a keyword-based computational measure comparing an online post to the scientific article’s abstract. We evaluate our measure using ground truth data labeled by within field experts. We highlight three main findings: first, we find a strong tendency towards low levels of information retention, following a distinct trajectory of loss except when bursts of attention begin in social media. Second, platforms show significant differences in information retention. Third, sequences involving more platforms tend to be associated with higher information retention. These findings highlight a strong tendency towards information loss over time—posing a critical concern for researchers, policymakers, and citizens alike—but suggest that multi-platform discussions may im-prove information retention overall. 

Retracted papers often circulate widely on social media, digital news, and other websites before their official retraction. The spread of potentially inaccurate or misleading results from retracted papers can harm the scientific community and the public. Here, we quantify the amount and type of attention 3,851 retracted papers received over time in different online platforms. Comparing with a set of nonretracted control papers from the same journals with similar publication year, number of coauthors, and author impact, we show that retracted papers receive more attention after publication not only on social media but also, on heavily curated platforms, such as news outlets and knowledge repositories, amplifying the negative impact on the public. At the same time, we find that posts on Twitter tend to express more criticism about retracted than about control papers, suggesting that criticism-expressing tweets could contain factual information about problematic papers. Most importantly, around the time they are retracted, papers generate discussions that are primarily about the retraction incident rather than about research findings, showing that by this point, papers have exhausted attention to their results and highlighting the limited effect of retractions. Our findings reveal the extent to which retracted papers are discussed on different online platforms and identify at scale audience criticism toward them. In this context, we show that retraction is not an effective tool to reduce online attention to problematic papers. 

In the ciliate Tetrahymena thermophila, the BCD1 pattern-gene encodes a Beige-BEACH-domain protein that defines cortical organelle dimensions through regulated endocytic activity. Tetrahymena cells homozygous for a bcd1 loss-of-function mutation exhibit supernumerary cortical organelles including oral apparatuses, cytoprocts and contractile vacuole pores. Elements of the broadened cortical domain phenotype can be phenocopied by disrupting clathrin-mediated endocytosis, suggesting that exocytic membrane delivery is balanced by endocytic retrieval of cortical pattern determinants.. 

Topics in conversations depend in part on the type of interpersonal relationship between speakers, such as friendship, kinship, or romance. Identifying these relationships can provide a rich description of how individuals communicate and reveal how relationships influence the way people share information. Using a dataset of more than 9.6M dyads of Twitter users, we show how relationship types influence language use, topic diversity, communication frequencies, and diurnal patterns of conversations. These differences can be used to predict the relationship between two users, with the best predictive model achieving a macro F1 score of 0.70. We also demonstrate how relationship types influence communication dynamics through the task of predicting future retweets. Adding relationships as a feature to a strong baseline model increases the F1 and recall by 1% and 2%. The results of this study suggest relationship types have the potential to provide new insights into how communication and information diffusion occur in social networks. 

Topics in conversations depend in part on the type of interpersonal relationship between speakers, such as friendship, kinship, or romance. Identifying these relationships can provide a rich description of how individuals communicate and reveal how relationships influence the way people share information. Using a dataset of more than 9.6M dyads of Twitter users, we show how relationship types influence language use, topic diversity, communication frequencies, and diurnal patterns of conversations. These differences can be used to predict the relationship between two users, with the best predictive model achieving a macro F1 score of 0.70. We also demonstrate how relationship types influence communication dynamics through the task of predicting future retweets. Adding relationships as a feature to a strong baseline model increases the F1 and recall by 1% and 2%. The results of this study suggest relationship types have the potential to provide new insights into how communication and information diffusion occur in social networks.