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Phase Separation in Biology and Disease; Current Perspectives and Open Questions

https://doi.org/10.1016/j.jmb.2023.167971

Boeynaems, Steven; Chong, Shasha; Gsponer, Jörg; Holt, Liam; Milovanovic, Dragomir; Mitrea, Diana M.; Mueller-Cajar, Oliver; Portz, Bede; Reilly, John F.; Reinkemeier, Christopher D.; et al (March 2023, Journal of Molecular Biology)

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Enhancer Features that Drive Formation of Transcriptional Condensates

https://doi.org/10.1016/j.molcel.2019.07.009

Shrinivas, Krishna; Sabari, Benjamin R.; Coffey, Eliot L.; Klein, Isaac A.; Boija, Ann; Zamudio, Alicia V.; Schuijers, Jurian; Hannett, Nancy M.; Sharp, Phillip A.; Young, Richard A.; et al (August 2019, Molecular Cell)
null (Ed.)
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Mediator Condensates Localize Signaling Factors to Key Cell Identity Genes

https://doi.org/10.1016/j.molcel.2019.08.016

Zamudio, Alicia V.; Dall’Agnese, Alessandra; Henninger, Jonathan E.; Manteiga, John C.; Afeyan, Lena K.; Hannett, Nancy M.; Coffey, Eliot L.; Li, Charles H.; Oksuz, Ozgur; Sabari, Benjamin R.; et al (December 2019, Molecular Cell)
null (Ed.)
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Pol II phosphorylation regulates a switch between transcriptional and splicing condensates

https://doi.org/10.1038/s41586-019-1464-0

Guo, Yang Eric; Manteiga, John C.; Henninger, Jonathan E.; Sabari, Benjamin R.; Dall’Agnese, Alessandra; Hannett, Nancy M.; Spille, Jan-Hendrik; Afeyan, Lena K.; Zamudio, Alicia V.; Shrinivas, Krishna; et al (August 2019, Nature)
null (Ed.)
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Transcription Factors Activate Genes through the Phase-Separation Capacity of Their Activation Domains

https://doi.org/10.1016/J.CELL.2018.10.042

Boija, Ann; Klein, Isaac A.; Sabari, Benjamin R.; Dall’Agnese, Alessandra; Coffey, Eliot L.; Zamudio, Alicia V.; Li, Charles H.; Shrinivas, Krishna; Manteiga, John C.; Hannett, Nancy M.; et al (December 2018, Cell)
null (Ed.)
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Partitioning of cancer therapeutics in nuclear condensates

https://doi.org/10.1126/science.aaz4427

Klein, Isaac A.; Boija, Ann; Afeyan, Lena K.; Hawken, Susana Wilson; Fan, Mengyang; Dall'Agnese, Alessandra; Oksuz, Ozgur; Henninger, Jonathan E.; Shrinivas, Krishna; Sabari, Benjamin R.; et al (June 2020, Science)
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The nucleus contains diverse phase-separated condensates that compartmentalize and concentrate biomolecules with distinct physicochemical properties. Here, we investigated whether condensates concentrate small-molecule cancer therapeutics such that their pharmacodynamic properties are altered. We found that antineoplastic drugs become concentrated in specific protein condensates in vitro and that this occurs through physicochemical properties independent of the drug target. This behavior was also observed in tumor cells, where drug partitioning influenced drug activity. Altering the properties of the condensate was found to affect the concentration and activity of drugs. These results suggest that selective partitioning and concentration of small molecules within condensates contributes to drug pharmacodynamics and that further understanding of this phenomenon may facilitate advances in disease therapy.
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Coactivator condensation at super-enhancers links phase separation and gene control

https://doi.org/10.1126/science.aar3958

Sabari, Benjamin R.; Dall’Agnese, Alessandra; Boija, Ann; Klein, Isaac A.; Coffey, Eliot L.; Shrinivas, Krishna; Abraham, Brian J.; Hannett, Nancy M.; Zamudio, Alicia V.; Manteiga, John C.; et al (July 2018, Science)