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Wheat (Triticum aestivumL.) resistance to Hessian fly (HF,Mayetiola destructor) conferred by HF resistance (R) genes, is often vulnerable to heat stress. This study aims to investigate the molecular basis of heat-induced susceptibility in wheat to HF. We compared the resistant cultivar ‘Molly’, which carriesH13, with its susceptible near-isogenic line ‘Newton’ to determine how elevated temperature alters phenotypic and molecular responses to HF infestation. Our phenotyping results showed that a single 24 h heat treatment at 30 °C was sufficient to compromise Molly’s resistance, resulting in more than 70% of plants becoming susceptible. Transcriptomic profiling revealed that resistant Molly exhibited stronger and broader defenses than Newton under normal temperature, whereas heat-stressed Molly displayed extensive transcriptional reprogramming resembling the naturally susceptible Newton wheat. Comparative analysis of transcriptomic profiles identified 74 genes that are consistently regulated across all susceptible states at 24 h after initial HF infestation, including heat-stressed Molly and Newton under both high and normal temperatures, but not in the resistant Molly under normal temperature. Functional annotation of these susceptibility-related genes in combination with previous findings suggest that susceptibility is likely associated with increased auxin-related activity, reduced salicylic acid (SA) and OPDA-associated defense signaling, and altered coordination of defense pathways that favor feeding-site establishment and redirecting host resource to developing HF larvae. The identification of susceptibility-associated candidate genes provides molecular targets for breeding wheat cultivars with more durable resistance under rising temperatures.more » « lessFree, publicly-accessible full text available May 5, 2027
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Free, publicly-accessible full text available May 5, 2027
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A sustainable and robust supply chain of rare earth elements (REEs) is necessary to meet our consumer, national security and clean energy goals. However, current intra-REE separation technologies (e.g.solvent extraction) are costly and carry a heavy environmental burden. Therefore, the development of new aqueous based ligands that are selective for individual REEs will be integral in future REE production systems. To develop these ligands, an understanding of how ligand coordination structure relates to selectivity is imperative. We used X-ray absorption spectroscopy (XAS) to observe the local structure around four lanthanide (Ln) ions (La, Ce, Pr and Nd) complexed by water and several relevant chelating ligands [lanmodulin EF-hand 1 peptides (LanM1), ethylenediaminetetraacetic acid (EDTA), aminotris(methylenephosphonic acid) (ATMP) and citric acid]. To collect these liquid-phase XAS spectra, we developed a new flow cell that prevents bubble interference and beam damage to the samples. In the X-ray absorption near-edge structure (XANES), we observed energy shifts in the white line, white line broadening and differences in the white line intensity of different Ln–ligand complexes between ligands. In the extended X-ray absorption fine structure (EXAFS), we distinguished differences in peak intensity and distance between coordinating ligands. Differences in the local coordination structure between Ln–LanM1 peptide complexes were more subtle compared with the other ligands (La–water, La–EDTA, La–ATMP and La–citric acid complexes). Further XANES and EXAFS studies, in combination with modelling and other techniques, could greatly improve our structural knowledge of how these aqueous ligands bind Ln ions and how they can be used to design more selective ligands for more efficient and sustainable REE separations.more » « lessFree, publicly-accessible full text available November 1, 2026
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Abstract All‐solid‐state batteries have the potential for enhanced safety and capacity over conventional lithium ion batteries, and are anticipated to dominate the energy storage industry. As such, strategies to enable recycling of the individual components are crucial to minimize waste and prevent health and environmental harm. Here, we use cold sintering to reprocess solid‐state composite electrolytes, specifically Mg and Sr doped Li7La3Zr2O12with polypropylene carbonate (PPC) and lithium perchlorate (LLZO−PPC−LiClO4). The low sintering temperature allows co‐sintering of ceramics, polymers and lithium salts, leading to re‐densification of the composite structures with reprocessing. Reprocessed LLZO−PPC−LiClO4exhibits densified microstructures with ionic conductivities exceeding 10−4 S/cm at room temperature after 5 recycling cycles. All‐solid‐state lithium batteries fabricated with reprocessed electrolytes exhibit a high discharge capacity of 168 mA h g−1at 0.1 C, and retention of performance at 0.2 C for over 100 cycles. Life cycle assessment (LCA) suggests that recycled electrolytes outperforms the pristine electrolyte process in all environmental impact categories, highlighting cold sintering as a promising technology for recycling electrolytes.more » « less
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Abstract We present the in-lab and on-sky performance for the upgraded 90 GHz focal plane of the Cosmology Large Angular Scale Surveyor, which had four of its seven detector wafers updated during the austral winter of 2022. The update aimed to improve the transition-edge-sensor (TES) stability and bias range and to realize the high optical efficiency of the sensor design. Modifications included revised circuit terminations, electrical contact between the TES superconductor and the normal metal providing the bulk of the bolometer heat capacity, and additional filtering on the TES bias lines. The upgrade was successful: 94% of detectors are stable down to 15% of the normal resistance, providing a wide overlapping range of bias voltages for all TESs on a wafer. The median telescope efficiency improved from to (68% quantiles). For the four upgraded wafers alone, median telescope efficiency increased to . Given our efficiency estimate for the receiver optics, this telescope efficiency implies a detector efficiency exceeding 0.90. The overall noise-equivalent temperature of the 90 GHz focal plane improved from to .more » « less
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Zmuidzinas, Jonas; Gao, Jian-Rong (Ed.)Front-end polarization modulation enables improved polarization measurement stability by modulating the targeted signal above the low-frequency $1/f$ drifts associated with atmospheric and instrumental instabilities and diminishes the impact of instrumental polarization. In this work, we present the design and characterization of a new 60-cm diameter Reflective Half-Wave Plate (RHWP) polarization modulator for the 90 GHz band telescope of the Cosmology Large Angular Scale Surveyor (CLASS) project. The RHWP consists of an array of parallel wires (diameter 50~µm, 175~µm pitch) positioned 0.88~mm from an aluminum mirror. In lab tests, it was confirmed that the wire resonance frequency ($$f_\mathrm{res}$$) profile is consistent with the target, $139$~Hz$$<154$$~Hz in the optically active region (diameter smaller than 150~mm), preventing the wire vibration during operation and reducing the RHWP deformation under the wire tension. The mirror tilt relative to the rotating axis was controlled to be $<15''$, corresponding to an increase in beam width due to beam smearing of < $0.6''$, %a beam smearing amplitude of $<0.6''$, negligible compared to the beam's full-width half-maximum of $36'$. The median and 16/84th percentile of the wire--mirror separation residual was $$0.048^{+0.013}_{-0.014}$$~mm in the optically active region, achieving a modulation efficiency $$\epsilon=96.2_{+0.5}^{-0.4}\%$$ with an estimated bandpass of 34~GHz. The angular velocity of the RHWP was maintained to an accuracy of within 0.005\% at the nominal rotation frequency (2.5~Hz). The RHWP has been successfully integrated into the CLASS 90 GHz telescope and started taking data in June 2024, replacing the previous modulator that has been in operation since June 2018.more » « less
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