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Abstract Plants have the ability to transmit mutations to progeny that arise through both meiotic and mitotic (somatic) cell divisions. This is because the same meristem cells responsible for vegetative growth will also generate gametes for sexual reproduction. Despite the potential for somatic mutations to contribute to genetic variation and adaptation, their role in plant evolution remains largely unexplored. We conducted experiments with the bush monkeyflower (Mimulus aurantiacus) to assess the phenotypic effects of somatic mutations inherited across generations. By generating self-pollinations within a flower (autogamy) or between flowers on different stems of the same plant (geitonogamy), we tracked the effects of somatic mutations transmitted to progeny. Autogamy and geitonogamy lead to different segregation patterns of somatic mutations among stems, with only autogamy resulting in offspring that are homozygous for somatic mutations specific to that stem. This allowed us to compare average phenotypic differences between pollination treatments that could be attributed to the inheritance of somatic variants. While most experimental units showed no impacts on fitness, in some cases, we detected increased seed production, as well as significant increases in drought tolerance, even though M. aurantiacus is already well adapted to drought conditions. We also found increased variance in drought tolerance following autogamy, consistent with the hypothesis that somatic mutations transmitted between generations can impact fitness. These results highlight the potential role of inherited somatic mutations as a relevant source of genetic variation in plant evolution. 

ABSTRACT Hybridisation is a common feature of evolutionary radiations, but its genomic consequences vary depending on when it occurs. Since reproductive isolation takes time to accumulate, hybridisation can occur at multiple points during divergence. Previous studies suggested that the taxonomic diversity in evolutionary radiations can help infer the timing of past gene flow events. Here, we assess the power of these approaches for revealing when gene flow occurred between two monkeyflower taxa (Mimulus aurantiacus) endemic to the Channel Islands of California. Coalescent simulations reveal that conventional four‐taxon tests may not be capable of fully distinguishing between recent and ancient introgression, but genome‐wide patterns of phylogenetic discordance vary predictably with different histories of hybridisation. Using whole‐genome sequencing and phylogenetic tests for introgression across theM. aurantiacusradiation, we identify signals of both ancient and recent hybridisation that occurred between the island taxa and their ancestors. In addition, we find widespread selection against introgressed ancestry, consistent with polygenic barriers to gene flow. However, we also identify localised signals across the genome that may indicate adaptive introgression. This study highlights the power and challenges of trying to disentangle complex histories of hybridisation. More broadly, our results illustrate the multiple roles that gene flow can play in evolutionary radiations: hybridisation can expose genetic incompatibilities that contribute to reproductive isolation while also likely facilitating adaptation by transferring beneficial alleles between taxa. These findings underscore the dynamic interplay between the timing of hybridisation and natural selection in shaping evolutionary trajectories within radiations.