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Ancient biomolecules have become an increasingly important part of archaeological investigations interested in understanding population movements and health. Despite their ability to elucidate historically-attested contexts of human mobility and interaction between different cultural groups, biomolecular techniques are still underutilized in certain historical and archaeological contexts. One such context is the Roman Imperial limes, or border zone, along the lower reaches of the Danube, which saw more than five hundred years of migration, conflict, and accommodation among a wide range of populations, from Mediterranean settlers to steppe pastoralists. In this region, more than a century of archaeological investigation has unearthed the remains of tens of thousands of Roman-era individuals. However, only a limited number of contexts have undergone biomolecular analyses. While these deceased humans may offer an untapped reservoir of biomolecular information, many were collected during a period when the standard precautions and protocols for ancient biomolecular research were not yet established. Because contamination is a major barrier for successfully recovering ancient DNA and proteins, conducting a pilot study to assess bimolecular preservation of a small representative dataset of human remains before embarking on a more extensive research program may prevent unnecessary sampling. This study applies ancient DNA and paleoproteomic techniques to human remains from a Roman-period cemetery at Histria, a site located just south of the Danube at the edge of the Roman province of Moesia Inferior. The individuals from whom we sampled dentin and dental calculus were excavated between the 1940s and 1980s and were housed at the Francisc J. Rainer Institute since. Our results suggest that both microbial and human ancient DNA is preserved in the dental calculus and dentin samples. We also successfully recovered sex-specific amelogenin peptides in tooth enamel from three individuals, including a juvenile. In conclusion, our results are encouraging, signifying the feasibility of future aDNA and paleoproteomic research for this skeletal collection. Our analyses also showcase how sex estimation with genomic and proteomic methods may contradict traditional osteological approaches. These findings not only offer deeper insights into the lives of these individuals but also show promise for the investigation of broader anthropological questions, such as the impact of Roman annexation in this region. 

The accelerating pace of emerging zoonotic diseases in the twenty-first century has motivated cross-disciplinary collaboration on One Health approaches, combining microbiology, veterinary and environmental sciences, and epidemiology for outbreak prevention and mitigation. Such outbreaks are often caused by spillovers attributed to human activities that encroach on wildlife habitats and ecosystems, such as land use change, industrialized food production, urbanization and animal trade. While the origin of anthropogenic effects on animal ecology and biogeography can be traced to the Late Pleistocene, the archaeological record—a long-term archive of human–animal–environmental interactions—has largely been untapped in these One Health approaches, thus limiting our understanding of these dynamics over time. In this review, we examine how humans, as niche constructors, have facilitated new host species and ‘disease-scapes’ from the Late Pleistocene to the Anthropocene, by viewing zooarchaeological, bioarchaeological and palaeoecological data with a One Health perspective. We also highlight how new biomolecular tools and advances in the ‘-omics’ can be holistically coupled with archaeological and palaeoecological reconstructions in the service of studying zoonotic disease emergence and re-emergence.

 

Recent research conducted in southern Sonora, Mexico provides an opportunity to revisit debates about interaction between Mesoamerica and the North American Southwest (NAS). In the borderland between these traditions, communities show few signs of cultural amalgamation, instead exhibiting either an avoidance of overt identity markers or an emphasis on more local connections. This pattern contrasts with most discussions of Mesoamerican influence on the NAS that focus on regionally atypical centers of foreign goods consumption or evidence of foreign religious traditions in distant localities. By recentering on local contexts where cultural amalgamation is expected but minimal, we raise important questions about why more distant groups found Mesoamerican societies to be worthy of emulation. The results suggest researchers should devote equal attention to cases in which distinct identities are erased or suppressed as they do to cases in which social boundaries are maintained or created anew.