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Focal ratio degradation (FRD), the decrease of light’s focal ratio between the input into an optical fiber and the output, is important to characterize for astronomical spectrographs due to its effects on throughput and the point spread function. However, while FRD is a function of many fiber properties such as stresses, microbending, and surface imperfections, angular misalignments between the incoming light and the face of the fiber also affect the light profile and complicate this measurement. A compact experimental setup and a model separating FRD from angular misalignment was applied to a fiber subjected to varying stresses or angular misalignments to determine the magnitude of these effects. The FRD was then determined for a fiber in a fiber positioner that will be used in the Subaru Prime Focus Spectrograph (PFS). The analysis we carried out for the PFS positioner suggests that effects of angular misalignment dominate and no significant FRD increase due to stress should occur.