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This dataset is for the following study: Anomalous crystalline ordering of particles in a viscoelastic fluid under high shear by Sijie Sun, Nan Xue, Stefano Aime, Hyoungsoo Kim, Jizhou Tang, Gareth H. McKinley, Howard A. Stone, and David A. Weitz. In this research, the authors investigate the high-shear-rate behavior of particle suspensions in viscoelastic fluids using a fully immersed parallel plate geometry. They discover an unexpected particle separation within the suspension, leading to the formation of a crystalline layer in the center of the cell. This solid layer disrupts the flow instability and introduces a new single-frequency component to the torque fluctuations, reflecting a dominant velocity pattern in the flow. The findings reveal the complex interplay between particles and the suspending viscoelastic fluid under extreme shear conditions. The SI videos, raw data and code of postprocessing are includedmore » « less
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Wang, Pai ; Zheng, Yue ; Fernandes, Matheus C. ; Sun, Yushen ; Xu, Kai ; Sun, Sijie ; Kang, Sung Hoon ; Tournat, Vincent ; Bertoldi, Katia ( , Physical Review Letters)
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Wadhwa, Neal ; Chen, Justin G. ; Sellon, Jonathan B. ; Wei, Donglai ; Rubinstein, Michael ; Ghaffari, Roozbeh ; Freeman, Dennis M. ; Büyüköztürk, Oral ; Wang, Pai ; Sun, Sijie ; et al ( , Proceedings of the National Academy of Sciences)
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Wang, Yongcheng ; Cao, Ting ; Ko, Jina ; Shen, Yinan ; Zong, Will ; Sheng, Kuanwei ; Cao, Wenjian ; Sun, Sijie ; Cai, Liheng ; Zhou, Ying‐Lin ; et al ( , Advanced Science)
Abstract Droplet‐based single cell sequencing technologies, such as inDrop, Drop‐seq, and 10X Genomics, are catalyzing a revolution in the understanding of biology. Barcoding beads are key components for these technologies. What is limiting today are barcoding beads that are easy to fabricate, can efficiently deliver primers into drops, and thus achieve high detection efficiency. Here, this work reports an approach to fabricate dissolvable polyacrylamide beads, by crosslinking acrylamide with disulfide bridges that can be cleaved with dithiothreitol. The beads can be rapidly dissolved in drops and release DNA barcode primers. The dissolvable beads are easy to synthesize, and the primer cost for the beads is significantly lower than that for the previous barcoding beads. Furthermore, the dissolvable beads can be loaded into drops with >95% loading efficiency of a single bead per drop and the dissolution of beads does not influence reverse transcription or the polymerase chain reaction (PCR) in drops. Based on this approach, the dissolvable beads are used for single cell RNA and protein analysis.