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In this presentation, we will do a longitudinal comparison of science lesson plan implementations from a group of preservice teachers’ experiences during a STEM-based summer program to their experiences during their Fall semester in their practice in regular elementary and middle schools. On the one hand, their summer experiences consisted of learning and implementing science and engineering lesson plans using culturally and linguistically sustaining pedagogies, which was an intensive and guided opportunity led by university faculty on one of the university campuses. In this experience, preservice teachers collaborated with peers for 15 days to implement and evaluate their teaching of science activities in a flexible environment. On the other hand, preservice teachers have their required practice in schools during senior year to implement lesson plans and become familiar with the regular tasks of an in-service teacher. This comparison is part of the research conducted by the Culturally Sustaining Pedagogies in Science for English Language Learners project funded by the National Science Foundation and focuses on providing the necessary pedagogical tools to teach STEM to multilingual students (in our case, from Latin American countries). We conclude with a series of recommendations for preservice teachers and in-service teachers who have multilingual and emerging bilingual learners in their classrooms.