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Feline urine provides valuable information on an animal’s wellbeing, but professional veterinary collection and analysis of urine samples can be intrusive, costly, and infrequent. Electrochemical biosensors recognize biological elements such as pH, glucose and sodium, and have numerous applications, including in medical diagnosis, environmental monitoring, food quality control and drug discovery. This paper presents cirCAT: PURRtentio, a litter box system that uses a electrochemical biosensor to monitor analytes in feline urine. We provide the implementation process of the system that consists of a DIY three-electrode biosensor, a potentiostat, a microcontroller, a ToF sensor and a mobile application. A rinsing mechanism is also included to extend the lifespan of the sensors. The system was tested using three separate electrochemistry tests to ensure accuracy, reliability, and applicability. We prepared and compared electrochemical biosensors with different conductive materials for Do-It-Yourself (DIY) electrodes. The second test compared PURRtentio against an industry-grade potentiostat. The third test compared our system against current veterinary standards for chemical analysis using feline’s urine samples. Additionally, we conducted a case study with a cat using PURRtentio for 72 hours. Finally, with results from these research and another series of interviews we did with veterinarian experts, we provide implications and future directions of this technology. PURRtentio presents an innovative and non-invasive means to consistently monitor chemistry elements in feline urine, potentially allowing for early detection and management of cat’s health conditions. 

Traditional collection and analysis of feline urine samples for health monitoring are invasive, expensive, and infrequent. This paper introduces PURRtentio, a novel litter box system utilizing an electrochemical biosensor to monitor analytes in feline urine. The system comprises a DIY biosensor, potentiostat, microcontroller, distance sensor, and mobile application. Performance validation compared PURRtentio with an industry-grade potentiostat. PURRtentio presents an innovative and non-invasive approach for consistent monitoring of chemistry elements in feline urine, enabling early detection and management of cat’s health conditions. This technology has the potential to revolutionize feline health monitoring, providing a solution for veterinarians and pet owners.