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As the interface between plants and soil, the organic horizon is the foundation of forest ecosystems. Two potential predictors of O-layer properties, vegetation and mineral soil type, are difficult to separate because they typically covary. We conducted a factorial study involving four canopy tree species and two soil types with distinctly different hydrology and topographic position to parse patterns in chemistry and microbiota of the O-layer in a north-temperate deciduous forest. These data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the USDA Forest Service, Northern Research Station.more » « less
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