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  1. Abstract

    Although communicating research is a key part of public science, current graduate curricula in the agricultural sciences usually have a narrow focus on communication appropriate for presenting to scientific and academic audiences, such as in the form of the dreaded “seminar.” Yet the importance and impact of agriculture extends well beyond research communities, and communicating with other potential audiences is essential for realizing the full impact of research. Because public speaking is among the greatest fears for many people, it is critical to provide students with the tools needed to communicate effectively with diverse audiences, particularly as only a fraction of them will go on to give regular research seminars once they enter the professional world. Better communication can lead to more constructive engagement with the public as well as with policy‐makers, toward improved understanding of the science they are funding and from which they are benefiting. Purposeful instruction in public speaking should help alleviate the common anxieties that student presenters often experience. Here, we summarize general communication strategies that can be incorporated into any graduate agricultural science course to help address this need.

     
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  2. Abstract

    As sequencing and genotyping technologies evolve, crop genetics researchers accumulate increasing numbers of genomic data sets from various genotyping platforms on different germplasm panels. Imputation is an effective approach to increase marker density of existing data sets toward the goal of integrating resources for downstream applications. While a number of imputation software packages are available, the limitations to utilization for the rice community include high computational demand and lack of a reference panel. To address these challenges, we develop the Rice Imputation Server, a publicly available web application leveraging genetic information from a globally diverse rice reference panel assembled here. This resource allows researchers to benefit from increased marker density without needing to perform imputation on their own machines. We demonstrate improvements that imputed data provide to rice genome-wide association (GWA) results of grain amylose content and show that the major functional nucleotide polymorphism is tagged only in the imputed data set.

     
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  3. Summary

    Understanding the genetic and physiological basis of abiotic stress tolerance under field conditions is key to varietal crop improvement in the face of climate variability. Here, we investigate dynamic physiological responses to water stressin silicoand their relationships to genotypic variation in hydraulic traits of cotton (Gossypium hirsutum), an economically important species for renewable textile fiber production.

    In conjunction with an ecophysiological process‐based model, heterogeneous data (plant hydraulic traits, spatially‐distributed soil texture, soil water content and canopy temperature) were used to examine hydraulic characteristics of cotton, evaluate their consequences on whole plant performance under drought, and explore potential genotype × environment effects.

    Cotton was found to have R‐shaped hydraulic vulnerability curves (VCs), which were consistent under drought stress initiated at flowering. Stem VCs, expressed as percent loss of conductivity, differed across genotypes, whereas root VCs did not. Simulation results demonstrated how plant physiological stress can depend on the interaction between soil properties and irrigation management, which in turn affect genotypic rankings of transpiration in a time‐dependent manner.

    Our study shows how a process‐based modeling framework can be used to link genotypic variation in hydraulic traits to differential acclimating behaviors under drought.

     
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  4. Summary

    Trees may survive prolonged droughts by shifting water uptake to reliable water sources, but it is unknown if the dominant mechanism involves activating existing roots or growing new roots during drought, or some combination of the two.

    To gain mechanistic insights on this unknown, a dynamic root‐hydraulic modeling framework was developed that set up a feedback between hydraulic controls over carbon allocation and the role of root growth on soil–plant hydraulics. The new model was tested using a 5 yr drought/heat field experiment on an established piñon‐juniper stand with root access to bedrock groundwater.

    Owing to the high carbon cost per unit root area, modeled trees initialized without adequate bedrock groundwater access experienced potentially lethal declines in water potential, while all of the experimental trees maintained nonlethal water potentials. Simulated trees were unable to grow roots rapidly enough to mediate the hydraulic stress, particularly during warm droughts. Alternatively, modeled trees initiated with root access to bedrock groundwater matched the hydraulics of the experimental trees by increasing their water uptake from bedrock groundwater when soil layers dried out.

    Therefore, the modeling framework identified a critical mechanism for drought response that required trees to shift water uptake among existing roots rather than growing new roots.

     
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  5. Abstract

    HighCO2and high temperature have an antagonistic interaction effect on rice yield potential and present a unique challenge to adapting rice to projected future climates. Understanding how the differences in response to these two abiotic variables are partitioned across rice germplasm accessions may be key to identifying potentially useful sources of resilient alleles for adapting rice to climate change. In this study, we evaluated eleven globally diverse rice accessions under controlled conditions at two carbon dioxide concentrations (400 and 600 ppm) and four temperature environments (29 °C day/21 °C night; 29 °C day/21 °C night with additional heat stress at anthesis; 34 °C day/26 °C night; and 34 °C day/26 °C night with additional heat stress at anthesis) for a suite of traits including five yield components, five growth characteristics, one phenological trait, and four photosynthesis‐related measurements. Multivariate analyses of mean trait data from these eight treatments divide our rice panel into two primary groups consistent with the genetic classification ofINDICA/INDICA‐like andJAPONICApopulations. Overall, we find that the productivity of plants grown under elevated [CO2] was more sensitive (negative response) to high temperature stress compared with that of plants grown under ambient [CO2] across this diversity panel. We report differential response toCO2× temperature interaction forINDICA/INDICA‐like andJAPONICArice accessions and find preliminary evidence for the beneficial introduction of exotic alleles into cultivated rice genomic background. Overall, these results support the idea of using wild or currently unadapted gene pools in rice to enhance breeding efforts to secure future climate change adaptation.

     
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