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We investigated biogeochemistry along a 12-m hyporheic mesocosm that allowed for controlled testing of seasonal and spatial water quality changes along a flowpath with fixed geometry and constant flow rate. Water quality profiles of oxygen, carbon, and nitrogen were measured at 1-m intervals along the mesocosm over multiple seasons. dissolved oxygen (DO) and temperature profiles were monitored on 18 dates between May 2019 through August 2020. Grab samples to monitor profiles of carbon, nitrogen, and various other solutes along the mesocosm were collected in December 2019 and August 2020 to provide more comprehensive biogeochemical analyses at time points when the dissolved oxygen (DO) and temperature profiles were at or near the maximum seasonal differences. Mesocosm monitoring ceased abruptly due to the Holiday Farm Fire, which burned from September through October 2020, cutting off personnel access and electrical power to the mesocosm facility.more » « less
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The benthic biolayer is a shallow zone of reactive streambed sediments, widely believed to contribute disproportionately to whole‐stream reactions such as aerobic respiration and contaminant transformation. Quantifying the relative contribution of the biolayer to whole‐stream reactions remains challenging because it requires that hyporheic zone solute transport and reaction heterogeneity are explicitly captured within a single modeling framework. Here, we use field experiments and modeling to quantify the biolayer's aerobic reactivity relative to other stream compartments. We co‐injected and monitored several fluorescent tracers, including the reactive tracer resazurin, into a controlled experimental stream. We characterized reactive transport in the water column and at multiple depths in the hyporheic zone by fitting all data to a new mobile‐immobile model, using resazurin‐to‐resorufin conversion as an indicator of aerobic bioreactivity. Results show that the biolayer converted 8 times more resazurin to resorufin than all other stream compartments, and 80% of this conversion occurred within 2 reach advection times. This hotspot and hot moment behavior is attributed to the biolayer's ability to rapidly acquire, transiently retain, and rapidly degrade stream‐borne solutes. The model analysis shows that the majority of raz‐to‐rru conversion occurs in the biolayer across streams with a wide range of biolayer structural properties, including streams with a biolayer that is less reactive than deeper regions of the hyporheic zone. Together, our results show that the biolayer is a common feature of streams and rivers that should be considered in network‐scale models of aerobic reactivity.more » « lessFree, publicly-accessible full text available November 1, 2026
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River corridors integrate the active channels, geomorphic floodplain and riparian areas, and hyporheic zone while receiving inputs from the uplands and groundwater and exchanging mass and energy with the atmosphere. Here, we trace the development of the contemporary understanding of river corridors from the perspectives of geomorphology, hydrology, ecology, and biogeochemistry. We then summarize contemporary models of the river corridor along multiple axes including dimensions of space and time, disturbance regimes, connectivity, hydrochemical exchange flows, and legacy effects of humans. We explore how river corridor science can be advanced with a critical zone framework by moving beyond a primary focus on discharge-based controls toward multi-factor models that identify dominant processes and thresholds that make predictions that serve society. We then identify opportunities to investigate relationships between large-scale spatial gradients and local-scale processes, embrace that riverine processes are temporally variable and interacting, acknowledge that river corridor processes and services do not respect disciplinary boundaries and increasingly need integrated multidisciplinary investigations, and explicitly integrate humans and their management actions as part of the river corridor. We intend our review to stimulate cross-disciplinary research while recognizing that river corridors occupy a unique position on the Earth's surface.more » « less
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The protection of headwater streams faces increasing challenges, exemplified by limited global recognition of headwater contributions to watershed resiliency and a recent US Supreme Court decision limiting federal safeguards. Despite accounting for ~77% of global river networks, the lack of adequate headwaters protections is caused, in part, by limited information on their extent and functions—in particular, their flow regimes, which form the foundation for decision-making regarding their protection. Yet, headwater streamflow is challenging to comprehensively measure and model; it is highly variable and sensitive to changes in land use, management and climate. Modelling headwater streamflow to quantify its cumulative contributions to downstream river networks requires an integrative understanding across local hillslope and channel (that is, watershed) processes. Here we begin to address this challenge by proposing a consistent definition for headwater systems and streams, evaluating how headwater streamflow is characterized and advocating for closing gaps in headwater streamflow data collection, modelling and synthesis.more » « less
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