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  1. Sediment traps were deployed to assess the mass and composition (iron, manganese, total organic carbon, and total nitrogen) of settling particulates in the water column of two drinking water reservoirs—Beaverdam Reservoir and Falling Creek Reservoir, both located in Vinton, Virginia, USA. Sediment traps were deployed at two depths in each reservoir to capture both epilimnetic and hypolimnetic (total) sediment flux. The particulates were collected from the traps approximately fortnightly from April to December from 2018 to 2022, then filtered, dried, and analyzed for either iron and manganese or total organic carbon and total nitrogen. Beaverdam and Falling Creek are owned and operated by the Western Virginia Water Authority as primary or secondary drinking water sources for Roanoke, Virginia. The sediment trap dataset consists of logs detailing the sample filtering process, the mass of dried particulates from each filter, and the raw concentration data for iron (Fe) and manganese (Mn), total organic carbon (TOC) and total nitrogen (TN). The final products are the calculated downward fluxes of solid Fe, Mn, TOC and TN during the deployment periods. 
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  2. Abstract Lake ecosystems, as integrators of watershed and climate stressors, are sentinels of change. However, there is an inherent time-lag between stressors and whole-lake response. Aquatic metabolism, including gross primary production (GPP) and respiration (R), of stream–lake transitional zones may bridge the time-lag of lake response to allochthonous inputs. In this study, we used high-frequency dissolved oxygen data and inverse modeling to estimate daily rates of summer epilimnetic GPP and R in a nutrient-limited oligotrophic lake at two littoral sites located near different major inflows and at a pelagic site. We examined the relative importance of stream variables in comparison to meteorological and in-lake predictors of GPP and R. One of the inflow streams was substantially warmer than the other and primarily entered the lake’s epilimnion, whereas the colder stream primarily mixed into the metalimnion or hypolimnion. Maximum GPP and R rates were 0.2–2.5 mg O 2 L −1  day −1 (9–670%) higher at littoral sites than the pelagic site. Ensemble machine learning analyses revealed that > 30% of variability in daily littoral zone GPP and R was attributable to stream depth and stream–lake transitional zone mixing metrics. The warm-stream inflow likely stimulated littoral GPP and R, while the cold-stream inflow only stimulated littoral zone GPP and R when mixing with the epilimnion. The higher GPP and R observed near inflows in our study may provide a sentinel-of-the-sentinel signal, bridging the time-lag between stream inputs and in-lake processing, enabling an earlier indication of whole-lake response to upstream stressors. 
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  4. Abstract. Empirical evidence demonstrates that lakes and reservoirs are warming acrossthe globe. Consequently, there is an increased need to project futurechanges in lake thermal structure and resulting changes in lakebiogeochemistry in order to plan for the likely impacts. Previous studies ofthe impacts of climate change on lakes have often relied on a single modelforced with limited scenario-driven projections of future climate for arelatively small number of lakes. As a result, our understanding of theeffects of climate change on lakes is fragmentary, based on scatteredstudies using different data sources and modelling protocols, and mainlyfocused on individual lakes or lake regions. This has precludedidentification of the main impacts of climate change on lakes at global andregional scales and has likely contributed to the lack of lake water qualityconsiderations in policy-relevant documents, such as the Assessment Reportsof the Intergovernmental Panel on Climate Change (IPCC). Here, we describe asimulation protocol developed by the Lake Sector of the Inter-SectoralImpact Model Intercomparison Project (ISIMIP) for simulating climate changeimpacts on lakes using an ensemble of lake models and climate changescenarios for ISIMIP phases 2 and 3. The protocol prescribes lakesimulations driven by climate forcing from gridded observations anddifferent Earth system models under various representative greenhouse gasconcentration pathways (RCPs), all consistently bias-corrected on a0.5∘ × 0.5∘ global grid. In ISIMIP phase 2, 11 lakemodels were forced with these data to project the thermal structure of 62well-studied lakes where data were available for calibration underhistorical conditions, and using uncalibrated models for 17 500 lakesdefined for all global grid cells containing lakes. In ISIMIP phase 3, thisapproach was expanded to consider more lakes, more models, and moreprocesses. The ISIMIP Lake Sector is the largest international effort toproject future water temperature, thermal structure, and ice phenology oflakes at local and global scales and paves the way for future simulations ofthe impacts of climate change on water quality and biogeochemistry in lakes. 
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  5. Abstract

    The concentration of dissolved organic nitrogen (DON) is increasing in many northern hemisphere lakes, yet its use by phytoplankton and fate in the environment seldom have been quantified. We conducted 1 week, in situ, microcosm incubations across 25 lakes in northeastern North America to understand how DON, dissolved inorganic nitrogen (DIN), and dissolved inorganic phosphorus (P) affected phytoplankton biomass. In addition, we tested whether lakes were limited by single macronutrients (N or P) or colimited by both. Phytoplankton biomass in 80% of lakes responded similarly to DON and DIN additions. Of the lakes where N form produced differential responses, the majority of phytoplankton communities exhibited greater biomass accumulation with DON than DIN. Colimitation was the most common type of nutrient limitation among the study lakes, followed by P limitation. Limitation type shifted with N form in 40% of the study lakes, but without consistent patterns explaining how shifts occurred. Regardless of N form, lakes with watersheds more dominated by agriculture and higher total dissolved nitrogen (TDN) tended to show P‐limited phytoplankton responses, while lakes with less agricultural watersheds and lower TDN tended to show colimited phytoplankton responses. Finally, ambient TDN and total phosphorus (TP) nutrient concentrations were stronger predictors of limitation type than ambient TDN : TP ratios. The different contributions of DON and DIN to phytoplankton biomass in some of our study lakes suggest that DON loading from surrounding watersheds may be an overlooked component in predicting phytoplankton productivity and nutrient limitation dynamics in freshwater ecosystems.

     
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