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The green peach aphid gut contains host plant microRNAs identified by comprehensive annotation of Brassica oleracea small RNA data
Like all organisms, aphids, plant sap-sucking insects that house a bacterial endosymbiont called
Buchnera, are members of a species interaction network. Ecological interactions across such networks can result in phenotypic change in network members mediated by molecular signals, like microRNAs. Here, we interrogated small RNA data from the aphid, Myzus persicae, to determine the source of reads that did not map to the aphid or Buchneragenomes. Our analysis revealed that the pattern was largely explained by reads that mapped to the host plant, Brassica oleracea, and a facultative symbiont, Regiella. To start elucidating the function of plant small RNA in aphid gut, we annotated 213 unique B. oleraceamiRNAs; 32/213 were present in aphid gut as mature and star miRNAs. Next, we predicted targets in the B. oleraceaand M. persicaegenomes for these 32 plant miRNAs. We found that plant targets were enriched for genes associated with transcription, while the distribution of targets in the aphid genome was similar to the functional distribution of all genes in the aphid genome. We discuss the potential of plant miRNAs to regulate aphid gene expression and the mechanisms involved in processing, export and uptake of plant miRNAs by aphids.
Although many insects are associated with obligate bacterial endosymbionts, the mechanisms by which these host/endosymbiont associations are regulated remain mysterious. While micro
RNAs (mi RNAs) have been recently identified as regulators of host/microbe interactions, including host/pathogen and host/facultative endosymbiont interactions, the role mi RNAs may play in mediating host/obligate endosymbiont interactions is virtually unknown. Here, we identified conserved mi RNAs that potentially mediate symbiotic interactions between aphids and their obligate endosymbiont, Buchnera aphidicola. Using small RNAsequence data from Myzus persicaeand Acyrthosiphon pisum, we annotated 93 M. persicaeand 89 A. pisummi RNAs, among which 69 were shared. We found 14 mi RNAs that were either highly expressed in aphid bacteriome, the Buchnera‐housing tissue, or differentially expressed in bacteriome vs. gut, a non‐ Buchnera‐housing tissue. Strikingly, 10 of these 14 mi RNAs have been implicated previously in other host/microbe interaction studies. Investigating the interaction networks of these mi RNAs using a custom computational pipeline, we identified 103 mi RNA:: mRNAinteractions shared between M. persicaeand A. pisum. Functional annotation of the shared mRNAtargets revealed only two over‐represented cluster of orthologous group categories: amino acid transport and metabolism, and signal transduction mechanisms. Our work supports a role for mi RNAs in mediating host/symbiont interactions between aphids and their obligate endosymbiont Buchnera. In addition, our results highlight the probable importancemore »