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Various machine learning-assisted directed evolution (MLDE) strategies have been shown to identify high-fitness protein variants more efficiently than typical wet-lab directed evolution approaches. However, limited understanding of the factors influencing MLDE performance across diverse proteins has hindered optimal strategy selection for wet-lab campaigns. To address this, we systematically analyzed multiple MLDE strategies, including active learning and focused training using six distinct zeroshot predictors, across 16 diverse protein fitness landscapes. By quantifying landscape navigability with six attributes, we found that MLDE offers a greater advantage on landscapes which are more challenging for directed evolution, especially when focused training is combined with active learning. Despite varying levels of advantage across landscapes, focused training with zero-shot predictors leveraging distinct evolutionary, structural, and stability knowledge sources consistently outperforms random sampling for both binding interactions and enzyme activities. Our findings provide practical guidelines for selecting MLDE strategies for protein engineering. 

Speech is a fundamental aspect of human life, crucial not only for communication but also for cognitive, social, and academic development. Children with speech disorders (SD) face significant challenges that, if unaddressed, can result in lasting negative impacts. Traditionally, speech and language assessments (SLA) have been conducted by skilled speech-language pathologists (SLPs), but there is a growing need for efficient and scalable SLA methods powered by artificial intelligence. This position paper presents a survey of existing techniques suitable for automating SLA pipelines, with an emphasis on adapting automatic speech recognition (ASR) models for children’s speech, an overview of current SLAs and their automated counterparts to demonstrate the feasibility of AI-enhanced SLA pipelines, and a discussion of practical considerations, including accessibility and privacy concerns, associated with the deployment of AI-powered SLAs. 

Tailoring interactions between a target ion and polymer can lead to highly precise ion separations for ultrathin membranes.