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Musier-Forsyth, Karin (Ed.)RNA-binding proteins play crucial roles in various cellular functions, and contain abundant disordered protein regions. The disordered regions in RNA-binding proteins are rich in repetitive sequences, such as poly-K/R, poly-N/Q, poly-A, and poly-G residues. Our bioinformatic analysis identified a largely neglected repetitive sequence family we define as electronegative clusters (ENCs) that contain acidic residues and/or phosphorylation sites. The abundance and length of ENCs exceed other known repetitive sequences. Despite their abundance, the functions of ENCs in RNA-binding proteins are still elusive. To investigate the impacts of ENCs on protein stability, RNA-binding affinity, and specificity, we selected one RNA-binding protein, themore »Free, publicly-accessible full text available July 9, 2022
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Existing network planning models for electric vehicle (EV) services usually treat the battery swap and the on-board supercharging as two independent processes. This study makes an early attempt to design an EV charging network where battery swap and supercharging are jointly coordinated. The swap and supercharge processes are characterized by Erlang B and Erlang C priority queues, respectively. A strategic location-allocation model is formulated to optimize the station sites, battery stock level, and the number of superchargers at chosen sites. Three design criteria, namely, battery state-of-charge, maximum service time, and power grid constraint, are simultaneously taken into account. Meta-heuristics algorithmsmore »